G418-RO
Roche
G-418 Solution
solution, =98% (TLC), suitable for transfection
Synonym(s):
G 418 disulfate salt solution, O-2-Amino-2,7-dideoxy-Δ-glycero-α-Δ-glucoheptopyranosyl[1->4]-O-3-deoxy-4C-methyl-3-[methyl-amino]-β-L-arabinopyranosyl-Δ-streptamine, disulfate salt, neomycin, Antibiotic G418
About This Item
Recommended Products
Quality Level
assay
98% (TLC)
form
solution
mol wt
692.7 Da
packaging
pkg of 100 ml (04727894001 [5 x 20 ml, equivalent to 5 g])
pkg of 20 mL (04727878001 [equivalent to 1 g])
manufacturer/tradename
Roche
concentration
50 mg/mL
technique(s)
transfection: suitable
antibiotic activity spectrum
protozoa
mode of action
protein synthesis | inhibits
storage temp.
2-8°C
SMILES string
OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O
InChI
1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
InChI key
UHEPSJJJMTWUCP-NKCAIAFTSA-N
Looking for similar products? Visit Product Comparison Guide
Related Categories
General description
Application
G-418 is also used to eliminate contaminating fibroblasts from mixed cultures.
Biochem/physiol Actions
Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.
Features and Benefits
- Convenient sterile-filtered solution for immediate use – saves valuable time and effort, and avoids potential errors arising from the preparation of sterile working stock solution from G-418 powder.
- Consistent quality and potency – shows no lot-to-lot variation in its ability to select resistant cells.
- Function tested in a cell culture assay - ensures effectiveness.
- Very pure, as indicated by TLC and microbiological assays – allows you to use less antibiotic while producing greater selection pressure. Lack of contaminants means minimal toxicity.
- Cost effective, since the selected colonies are stable.
Aqueous solution of G-418, 892 μg/mg, filtered through 0.2μm pore size membrane
Quality
Function testing: in cell culture.
Preparation Note
Active weight: >700μg/mg
Recommended concentration in cell culture: The optimal concentration of G-418 must be determined experimentally; it varies from 100μg/ml to 1mg/ml, depending on the cell type used.
Specific rotation: 104° to 121°, in water (on dry basis, according to USP XXII).
Other Notes
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
No data available
flash_point_c
No data available
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Articles
In this Biochemica article, the dose-response curve of different concentrations of G-418 solution were tested for their ability to stop growth of the adherent PC-3 prostate cancer cell line. Please also refer to the package insert.
Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service