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04732

Supelco

2,4-Dinitrophenylhydrazine

for HPLC derivatization, LiChropur, moistened with 35% water, ≥99.0% (HPLC)

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About This Item

Linear Formula:
(O2N)2C6H3NHNH2
CAS Number:
Molecular Weight:
198.14
Beilstein/REAXYS Number:
615586
EC Number:
MDL number:
UNSPSC Code:
41116105
PubChem Substance ID:
NACRES:
NA.22

grade

for HPLC derivatization

Quality Level

assay

≥99.0% (HPLC)

quality

LiChropur
moistened with 35% water

technique(s)

HPLC: suitable

mp

197-200 °C (lit.)

SMILES string

NNc1ccc(cc1[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C6H6N4O4/c7-8-5-2-1-4(9(11)12)3-6(5)10(13)14/h1-3,8H,7H2

InChI key

HORQAOAYAYGIBM-UHFFFAOYSA-N

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Application

2,4-Dinitrophenylhydrazine may be used as a derivatization agent in the analysis of aldehydes and ketones using liquid chromatography coupled with atmospheric pressure photoionization-mass spectrometry (LC-APPI-MS).

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Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

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Determination of aldehydes and ketones using derivatization with 2, 4-dinitrophenylhydrazine and liquid chromatography?atmospheric pressure photoionization-mass spectrometry.
van Leeuwen SM, et al.
Journal of Chromatography A, 1058(1-2), 107-112 (2004)
The determination of dehydroascorbic acid and ascorbic acid in plant tissues by the 2, 4-dinitrophenylhydrazine method.
Roe JH, et al.
The Journal of Biological Chemistry, 152, 511-517 (1944)
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Light-grown Arabidopsis thaliana cell suspension culture (ACSC) were subjected to mild photooxidative damage with Rose Bengal (RB) with the aim of gaining a better understanding of singlet oxygen-mediated defence responses in plants. Additionally, ACSC were treated with H2O2 at concentrations
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Antioxidant administration aimed to antagonize the development and progression of disuse muscle atrophy provided controversial results. Here we investigated the effects of curcumin, a vegetal polyphenol with pleiotropic biological activity, because of its ability to upregulate glucose-regulated protein 94 kDa

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