04732
2,4-Dinitrophenylhydrazine
for HPLC derivatization, LiChropur™, moistened with 35% water, ≥99.0% (HPLC)
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About This Item
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grade
for HPLC derivatization
Quality Level
assay
≥99.0% (HPLC)
quality
LiChropur™
moistened with 35% water
technique(s)
HPLC: suitable
mp
197-200 °C (lit.)
SMILES string
NNc1ccc(cc1[N+]([O-])=O)[N+]([O-])=O
InChI
1S/C6H6N4O4/c7-8-5-2-1-4(9(11)12)3-6(5)10(13)14/h1-3,8H,7H2
InChI key
HORQAOAYAYGIBM-UHFFFAOYSA-N
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Application
2,4-Dinitrophenylhydrazine may be used as a derivatization agent in the analysis of aldehydes and ketones using liquid chromatography coupled with atmospheric pressure photoionization-mass spectrometry (LC-APPI-MS).
Legal Information
LiChropur is a trademark of Merck KGaA, Darmstadt, Germany
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Determination of aldehydes and ketones using derivatization with 2, 4-dinitrophenylhydrazine and liquid chromatography?atmospheric pressure photoionization-mass spectrometry.
Journal of Chromatography A, 1058(1-2), 107-112 (2004)
The determination of dehydroascorbic acid and ascorbic acid in plant tissues by the 2, 4-dinitrophenylhydrazine method.
The Journal of Biological Chemistry, 152, 511-517 (1944)
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