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11945

Supelco

Benedict′s reagent

for the qualitative determination of sugars

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About This Item

CAS Number:
63126-89-6
MDL number:
MFCD00146068
UNSPSC Code:
12164500
NACRES:
NA.21

Quality Level

100

technique(s)

photometry: suitable

impurities

cupric sulfate

application(s)

food and beverages
general analytical

InChI

1S/C6H8O7.CH2O3.Cu.2Na.H2O4S.H2O/c7-3(8)1-6(13,5(11)12)2-4(9)10;2-1(3)4;;;;1-5(2,3)4;/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);(H2,2,3,4);;;;(H2,1,2,3,4);1H2/q;;+2;2*+1;;/p-4

InChI key

QFKWSRIUZIYLCK-UHFFFAOYSA-J

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General description

Benedict′s reagents comprise of copper sulphate, sodium carbonate which functions as a mild alkali, and sodium citrate. It is quite stable. On boiling this reagent with glucose, it gives a white precipitate of cuprous thiocyanate. It is primarily used for the estimation of glucose in urine.

Application

Benedict′s reagent may be used during TLC analysis of mesocotyl methanol extracts during a study to evaluate sorghum resistance associated with accumulation of undetected toxic host metabolites. It may also be also be used to synthesize an SEM image of the cubic shaped Cu2O with smooth surface.

Other Notes

Reagent used for the detection of reducing sugars and other reducing substances; detection limit: 0.01 % glucose in water

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves

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R L Nicholson et al.
Proceedings of the National Academy of Sciences of the United States of America, 84(16), 5520-5524 (1987-08-01)
Infection of the sorghum mesocotyl by Helminthosporium maydis (a nonpathogen) and Colletotrichum graminicola (a pathogen) resulted in the rapid accumulation of a pigment complex by two sorghum cultivars. The components of the complex were fungitoxic. The principal compounds have been
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