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198218

Sigma-Aldrich

Patent Blue VF

Dye content 50 %

Synonym(s):

Acid Blue 1, Sulfan Blue

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About This Item

Empirical Formula (Hill Notation):
C27H31N2NaO6S2
CAS Number:
129-17-9
Molecular Weight:
566.66
Colour Index Number:
42045
EC Number:
204-934-1
MDL number:
MFCD00012119
UNSPSC Code:
12171500
PubChem Substance ID:
24851867
NACRES:
NA.47

form

powder

Quality Level

200

composition

Dye content, 50%

solubility

H2O: 1 mg/mL

λmax

635 nm (2nd)
-410 nm

ε (extinction coefficient)

≥50000 at 635-641 nm in H2O
≥5300 at 408-414 nm in H2O
≥7500 at 308-314 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].CCN(CC)c1ccc(cc1)\C(=C2/C=CC(\C=C2)=[N+](\CC)CC)c3ccc(cc3S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C27H32N2O6S2.Na/c1-5-28(6-2)22-13-9-20(10-14-22)27(21-11-15-23(16-12-21)29(7-3)8-4)25-18-17-24(36(30,31)32)19-26(25)37(33,34)35;/h9-19H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1

InChI key

SJEYSFABYSGQBG-UHFFFAOYSA-M

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Application

Patent Blue VF has been used:
  • in combination with 99mTc phytate to detect sentinel lymph node (SLN) in early stage cervical cancer patients.
  • to detect heat shock protein (HSP) gp96 induced draining lymph nodes (LNs) in mice.
  • to detect the effect of Asn-Gly-Arg (NGR)-tumor necrosis factor (TNF) on tumor perfusion in murine model.

Biochem/physiol Actions

Patent Blue VF dye is a violet colored powder. It has been used in cosmetic coloring.

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Synergistic antitumor activity of cisplatin, paclitaxel, and gemcitabine with tumor vasculature-targeted tumor necrosis factor-alpha.
Sacchi A
Clinical Cancer Research, 12(1), 175-182 (2006)
Sentinel lymph node detection in early cervical cancer with combination 99mTc phytate and patent blue.
Niikura H
Gynecologic Oncology, 94(2), 528-532 (2004)
Blue dyes in medicine--a confusing terminology.
Kathrin Scherer et al.
Contact dermatitis, 54(4), 231-232 (2006-05-03)
Cutting Edge: Heat Shock Protein gp96
Induces Maturation and Migration of
CD11c1 Cells In Vivo
Binder R J
Journal of Immunology, 165, 6029-6035 (2000)
Jeffrey How et al.
Gynecologic oncology, 127(2), 332-337 (2012-08-23)
The objective of this study is to evaluate the detection rate and diagnostic accuracy of sentinel lymph node (SLN) mapping using intra-operative cervical injection of filtered 99mTc-sulfur colloid (99mTc-SC) and patent blue in patients with endometrial cancer. Prospective evaluation of
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