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Tryptic Soy Broth - Dehydrated Culture Media

NutriSelect® Plus, powder, suitable for microbiology

Synonym(s):

CASO Broth, Soybean Casein digest Broth, TSB, Tryptone Soy Broth, CASO Broth, Casein Soya Broth, Soybean Casein digest Broth, TSB, Tryptone Soya Broth

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About This Item

MDL number:
MFCD00132536
UNSPSC Code:
41171607
NACRES:
NA.74

agency

according to ISO 10273:2017
according to ISO 21871:2006

Quality Level

200

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

casein peptone (pancreatic), 17 g/L
dipotassium hydrogen phosphate, 2.5 g/L
glucose, 2.5 g/L
sodium chloride, 5 g/L
soya peptone (papain digest.), 3 g/L

packaging

bottle of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

color

light yellow

final pH

7.3±0.2 (25 °C)

application(s)

bioburden testing
cleaning products
clinical testing
cosmetics
environmental
food and beverages
pathogen testing
personal care
pharmaceutical
sample preparation
veterinary
water monitoring
microbiology

suitability

nonselective for Bacillus spp.
nonselective for Bacteroides spp.
nonselective for Campylobacter spp.
nonselective for Candida spp.
nonselective for Escherichia coli
nonselective for Neisseria spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms

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General description

Tryptic Soy Broth is a general-purpose culture medium for the isolation and cultivation of fastidious and non-fastidious microorganisms or the maintenance of stock culture. It is a complex medium suitable for the cultivation and isolation of a wide range of bacteria, yeasts, and molds. The formulation of the TSB medium contains a combination of casein and soy peptones supplying organic nitrogen, particularly amino acids, and long-chained peptides. Sodium chloride maintains the osmotic equilibrium, and the natural sugars from soy peptone are the energy sources.

Application

Tryptic Soy Broth is a medium that supports the luxuriant growth of many fastidious organisms without adding serum. It is used for confirmation of Campylobacter jejuni by motility test. It is suitable for the cultivation of both aerobic and anaerobic bacteria.

Features and Benefits

NutriSelect® Plus are powdered culture media offering
  • Quality control with growth promotion test according to industry standards
  • Quality assurance acc. to ISO 9001
  • Cost-efficient powdered media

Preparation Note

Suspend 30 g of dehydrated media in 1 litre of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.

Other Notes

Capability of selective media to detect heat-injured Shigella flexneri

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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S K Urwyler et al.
Letters in applied microbiology, 62(2), 130-137 (2015-11-20)
Fast microbial identification is becoming increasingly necessary in industry to improve microbial control and reduce biocide consumption. We compared the performances of two systems based on MALDI-TOF MS (VITEK MS and BIOTYPER) and two based on biochemical testing (BIOLOG, VITEK
Lucia Hrncirova et al.
Folia microbiologica, 64(4), 497-508 (2019-01-19)
The aim of this work was to test the hypothesis that antimicrobial food additives may alter the composition of human gut microbiota by selectively suppressing the growth of susceptible gut microbes. To explore the influence of antimicrobial food additives on
H S Nagendra Prasad et al.
Microbial pathogenesis, 127, 106-115 (2018-12-07)
The synthesized potent piperazine analog ChDiPiCa was characterised by various spectroscopic techniques and for the first time evaluated functional membrane microdomain (FMM) disassembly in methicillin-resistant Staphylococcus aureus (MRSA). The ChDiPiCa showed excellent in vitro biocidal activity against MRSA at 26 μg/mL
K D Teague et al.
Poultry science, 96(7), 2074-2082 (2017-02-06)
Three experiments were conducted to evaluate the effect of in ovo administration of FloraMax®-B11 (FM) on Marek's disease (MD) herpesvirus of turkeys (HVT) vaccine protective efficacy, hatchability, microbiota composition, morphometric analysis, and Salmonella enteritidis (SE) infection in chickens. Experiment 1
Omar F Prado-Rebolledo et al.
Avian pathology : journal of the W.V.P.A, 46(1), 90-94 (2016-08-23)
Two experiments were conducted to evaluate the effect of a lactic acid bacteria-based probiotic (FloraMax-B11
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