Skip to Content
MilliporeSigma
All Photos(2)

Documents

30181

Supelco

Fluorescein sodium salt

analytical standard

Synonym(s):

Acid Yellow 73, D&C;Yellow No. 8, NaFl, NaFluo, Sodium fluorescein, Uranine

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

Empirical Formula (Hill Notation):
C20H10Na2O5
CAS Number:
518-47-8
Molecular Weight:
376.27
Colour Index Number:
45350
Beilstein/REAXYS Number:
3833041
EC Number:
208-253-0
MDL number:
MFCD00167039
UNSPSC Code:
85151701
PubChem Substance ID:
329753341
NACRES:
NA.24

grade

analytical standard

Quality Level

100

assay

≥97.5% (HPLC)

shelf life

limited shelf life, expiry date on the label

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

impurities

≤15% water

fluorescence

λex 460 nm; λem 515 nm(lit.)

format

neat

SMILES string

[Na+].[Na+].[O-]c1ccc2c(Oc3cc([O-])ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2

InChI key

RGPLGPBQJOQFJS-UHFFFAOYSA-L

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Fluorescein sodium salt (uranine) is a xanthene dye, which finds applications as a fluorescent tracer and shows the best of fluorescence in the pH range 6 to 11. Uranine is also employed as a coloring additive in drugs and cosmetics. It is known to exhibit optical chemical sensing and fluorescent properties in biological staining procedures.

Application

Fluorescein sodium salt is used as an analytical standard in determining the formation of hydroxy radicals, by the ZnO-based photocatalysts under UV-filtered simulated sunlight using spectrofluorometry. It is also used as a calibration standard for the label-free measurements of metabolic events within cells in the living animals using fluorescence lifetime imaging microscopy (FLIM).
It may also be used as an analytical reference standard for the quantification of the analyte in the following:
  • Soil samples using high-performance liquid chromatography (HPLC).
  • Coffee samples using high-performance liquid chromatography (HPLC) and capillary electrophoresis (CZE).

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Analysis Note

remarks on ICP: Na positive

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

423.7 °F - Pensky-Martens closed cup

flash_point_c

217.6 °C - Pensky-Martens closed cup

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 4

1 of 4

Fluorescein European Pharmacopoeia (EP) Reference Standard

Y0000796

Fluorescein

Fluorescein (free acid) Dye content 95 %

Sigma-Aldrich

F2456

Fluorescein (free acid)

Uranine AP (C.I. 45350) concentrated for the examination of subterranean waters

Supelco

1.08462

Uranine AP (C.I. 45350)

Indigo European Pharmacopoeia (EP) Reference Standard

Y0001752

Indigo

Solid-phase extraction (SPE) of the fluorescence tracers uranine and sulphorhodamine B
Franke C, et al.
Water Research, 31(10), 2633-2637 (1997)
Jitendra Wankar et al.
International journal of pharmaceutics, 531(2), 568-576 (2017-05-27)
Ethionamide (ETH) is a second line antitubercular drug suffering from poor solubility in water and strong tendency to crystallize. These drawbacks were addressed by loading ETH in β-cyclodextrin (βCyD)-based carriers. The drug was incorporated in a molecular state avoiding crystallization
Highly reduced ecotoxicity of ZnO-based micro/nanostructures on aquatic biota: Influence of architecture, chemical composition, fixation, and photocatalytic efficiency
Serra A, et al.
Water Research, 169(9), 115210-115210 (2020)
Method for the analysis of phloxine B, uranine, and related xanthene dyes in soil using supercritical fluid extraction and high-performance liquid chromatography
Alcantara-Licudine PJ, et al.
Journal of Agricultural and Food Chemistry, 45(3), 766-773 (1997)
Chenying Yang et al.
Journal of biomedical optics, 19(7), 76014-76014 (2014-07-17)
Fluorescence molecular imaging with exogenous probes improves specificity for the detection of diseased tissues by targeting unambiguous molecular signatures. Additionally, increased diagnostic sensitivity is expected with the application of multiple molecular probes. We developed a real-time multispectral fluorescence-reflectance scanning fiber
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service