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Sigma-Aldrich

Methanol

≥99.8%, anhydrous, suitable for tissue processing

Synonym(s):

Methyl alcohol

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About This Item

Linear Formula:
CH3OH
CAS Number:
67-56-1
Molecular Weight:
32.04
Beilstein/REAXYS Number:
1098229
EC Number:
200-659-6
MDL number:
MFCD00004595
UNSPSC Code:
12352104
PubChem Substance ID:
57648672

product name

Methanol, anhydrous, 99.8%

grade

anhydrous

Quality Level

200

vapor density

1.11 (vs air)

vapor pressure

410 mmHg ( 50 °C)
97.68 mmHg ( 20 °C)

assay

99.8%

form

liquid

autoignition temp.

725 °F

expl. lim.

36 %

technique(s)

tissue processing: suitable

impurities

<0.002% water
<0.005% water (100 mL pkg)

evapn. residue

<0.0003%

refractive index

n20/D 1.329 (lit.)

bp

64.7 °C (lit.)

mp

−98 °C (lit.)

density

0.791 g/mL at 25 °C (lit.)

format

neat

SMILES string

CO

InChI

1S/CH4O/c1-2/h2H,1H3

InChI key

OKKJLVBELUTLKV-UHFFFAOYSA-N

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General description

Methanol is an industrial organic solvent commonly used in organic synthesis. It is being considered as a potential hydrogen source in fuel cell technology due to its high H/C ratio, low propensity for soot generation, relatively low reforming temperature and its liquid state at room temperature. The olefins (ethylene or propylene) formed from methanol via MTO (methanol-to-olefins) process, can be an alternative to oil and gas to produce hydrocarbon fuels.

Application

Methanol has been used for fixing the tissues during preparation of thin giemsa stained blood smears. It has been used in the purification step during the synthesis of pyocyanin, a toxic compound found in Pseudomonas aeruginosa.
Methanol has been used in following applications:
  • Used for the synthesis and purification of pyocyanin, spectrophotometric estimation of pyocyanin concentration, and the calculation of pyocyanin molar concentration using spectrophotometer absorption data.
  • Used as a component of staining solution used in karyotyping.
  • Used as a component of staining solution to stain cell colonies in colony formation assay.

Packaging

View returnable container options.

Other Notes

Pure-Pac® II containers require the Micromatic MacroValve coupler for dispensing solvents, Z560723.

Legal Information

Pure-Pac is a registered trademark of Merck KGaA, Darmstadt, Germany

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signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

target_organs

Eyes,Central nervous system

wgk_germany

WGK 2

flash_point_f

closed cup

flash_point_c

closed cup

ppe

Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
STBL0086
STBL0002
STBL0001
STBK7023
STBK7015

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Generation of Embryonic Stem Cells in Rabbits.
Methods in Molecular Biology, 1341, 49-66 (2016)
Matthew P Horning et al.
Lab on a chip, 14(12), 2040-2046 (2014-05-02)
A paper microfluidic cartridge for the automated staining of malaria parasites (Plasmodium) with acridine orange prior to microscopy is presented. The cartridge enables simultaneous, sub-minute generation of both thin and thick smears of acridine orange stained parasites. Parasites are stained
Standardized chemical synthesis of Pseudomonas aeruginosa pyocyanin.
Cheluvappa R.
Gastroenterologia y hepatologia, 22, 1350-1351 (2007)
Rajkumar Cheluvappa
MethodsX, 1, 67-73 (2014-01-01)
Preparation of the toxin pyocyanin from the bacterium Pseudomonas aeruginosa is an exacting procedure. Pyocyanin is expensive to commercially purchase. The sellers do not give out the extraction procedure. Classically, pyocyanin preparation involves complicated multi-step P. aeruginosa culturing and solvent
Beyond oil and gas: the methanol economy.
Olah GA.
Angewandte Chemie (International Edition in English), 44(18), 2636-2639 (2005)
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