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Potato Dextrose Agar

NutriSelect® Plus, suitable for microbiology

Synonym(s):

PDA, Potato Glucose Agar, Potato Glucose Agar

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About This Item

UNSPSC Code:
41171605
NACRES:
NA.74

agency

APHA
USP

Quality Level

200

reg. compliance

FDA

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
dextrose, 20 g/L
potato extract, 4 g/L

packaging

pkg of 100 g
pkg of 2.5 kg
pkg of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

color

light yellow

final pH

5.6±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
microbiology

suitability

nonselective for Aspergillus spp.
nonselective for Candida spp.
nonselective for Penicillium spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.
nonselective for molds (General Media)
nonselective for yeasts (General Media)

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General description

Potato Dextrose Agar is recommended for the isolation and enumeration of yeasts and molds in dairy and food products. Potato extract serves as a source of carbon, nitrogen, minerals, vitamins, and other essential growth nutrients. Dextrose acts as a source of carbohydrates. Agar is the solidifying agent. The accompanying bacterial flora is suppressed by the low pH of 3.5. The growth of yeasts and molds is promoted in this medium, and the fungi develop typical morphology. The addition of Rose Bengal further suppresses the growth of bacteria and restricts the size and spreading of mold colonies.

Application

Potato Dextrose Agar is used for the isolation and enumeration of yeasts and molds in dairy and food products. The media is also used for stimulating sporulation, maintaining stock cultures of certain dermatophytes, and as a differential media for certain varieties of dermatophytes on the basis of pigment production.

Features and Benefits

NutriSelect® Plus are powdered culture media offering
  • Quality control with growth promotion test according to industry standards
  • Quality assurance acc. to ISO 9001
  • Cost-efficient powdered media

Preparation Note

Suspend 39 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. In order to suppress bacterial growth, 1 ml of 10% sterile lactic acid can be added for dropping pH to 3.5. Mix well before pouring.

Other Notes

Studies on contamination of yogurt by yeast; For maintaining the stock culture of Rhodotorula glutinis for the production of extracellular lipase; Identification of Aspergillus niger from infected onion tissue

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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R.E. Beever et al.
Journal of General Microbiology, 60, 273-273 (1970)
M. Dublin Green et al.
Journal of Food Protection, 50, 193-193 (1987)
A. Behere et al.
Enzyme and Microbial Technology, 15, 158-158 (1993)
D. Papaparaskevas et al.
Biotechnology Letters, 14, 397-397 (1992)
Julia Schumacher et al.
Eukaryotic cell, 7(4), 584-601 (2008-02-12)
Recently, we showed that the alpha subunit BCG1 of a heterotrimeric G protein is an upstream activator of the Ca(2+)/calmodulin-dependent phosphatase calcineurin in the gray mold fungus Botrytis cinerea. To identify the transcription factor acting downstream of BCG1 and calcineurin
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