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E. coli ChromoSelect Agar B

NutriSelect® Plus, suitable for microbiology

Synonym(s):

E. coli Agar B ChromoSelect

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
bile salt mixture, 1.5 g/L
casein enzymic hydrolysate, 20 g/L
X-glucuronide, 0.075 g/L

manufacturer/tradename

NutriSelect® Plus

final pH

7.2±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring
microbiology

suitability

selective and differential for Escherichia coli
selective and differential for Klebsiella spp.
selective and differential for coliforms

Related Categories

Application

E. coli ChromoSelect Agar B is recommended for the detection and enumeration of Escherichia coli in foods without further confirmation on membrane filter or by indole reagent. Most of the Escherichia coli strains can be differentiated from other coliforms by the presence of enzyme glucuronidase which is highly specific for Escherichia coli. Escherichia coli cells absorb X-glucuronide and the intracellular glucuronidase splits the bond between the chromophore and the glucuronide. The released chromophore gives the blue coloration of the colonies.

Preparation Note

Suspend 36.6 g per litre in distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour into sterile petri plates.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Articles

Overview of Chromogenic Media

Selective media enable faster results and visual confirmation for the detection, identification, and enumeration of microorganisms

Selective Growth Media for Differentiation and Detection of Escherichia coli and Other Coliforms

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service