71019BC
Assurance® GDS MPX ID for Top STEC Kit
suitable for PCR testing in Food and Beverages applications
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About This Item
UNSPSC Code:
41106302
NACRES:
NA.85
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product name
GDS MPX ID for Top STEC, BioControl, Molecular based secondary screening PCR test for the detection and identification of "Top Six" non-O157 Shiga Toxigenic E. coli (STEC) in food and environmental samples
agency
AOAC® (PTM)
Quality Level
shelf life
6‑12 mo.
packaging
pkg of 52 tests
manufacturer/tradename
Assurance GDS®
BioControl Systems 71019-52
technique(s)
PCR: suitable
application(s)
food and beverages
pathogen testing
compatibility
for use with GDS Rotor-Gene
storage temp.
2-8°C
suitability
enterovirulent E. coli (EEC) (shiga toxigenic)
Related Categories
General description
Assurance® GDS is an advanced genetic detection system for food pathogens. The MPX ID for Top STEC is a secondary automated nucleic acid amplification system for the identification of “Top Six” non-O157 Shiga Toxigenic E. coli (STEC). The Top 6 non-O157 STEC are defined as E. coli belonging to serogroups O103, O111, O121, O145, O26, or O45 that possess both the eae gene and at least one of the Shiga Toxin genes (stx1 or stx2). GDS MPX ID utilizes a proprietary PickPen® IMS-based sample preparation procedure to quickly and effectively capture organisms belonging to the 6 specific Top STEC O-serogroups prior to genetic analysis for the associated pathogenicity genes. Each of the 3 unique eae gene sequences targeted by MPX ID are highly correlated plus one or more of the 6 O-serogroups comprising Top 6 non-O157 STEC. Because there are 3 different eae gene targets and 6 different O-serogroup targets, the PCR reactions have been divided between 2 Amplification Tubes. Each tube contains an internal control and all necessary PCR reagents. Results will automatically be combined between the 2 tubes via GDS Rotor-Gene® software.
Application
Assurance® GDS MPX ID for Top STEC is designed to follow GDS MPX Top 7 STEC (PN 71015BC) for the identification of “Top Six” non-O157 Shiga Toxigenic E. coli (STEC) in a variety of foods, ingredients, and environmental samples. Additionally, GDS MPX ID can be used to confirm isolated colonies from samples presumptive positive for Top 6 STEC.
Features and Benefits
- Designed specifically to follow GDS MPX Top STEC assays
- Identification of the specific O serogroup(s) responsible for the positive STEC result and verifies that all targets relevant to the definition of a Top 6 STEC (eae, stx and O group) are present within a single organism
- Confirm isolated colonies from samples presumptive positive for Top 6 STEC
- Utilizes the same single-step mEHEC® enrichment media common to all BioControl methods for Salmonella, E.coli O157:H7, Shiga Toxin genes, and Top STEC
- Only 14 hours from start to finish
Components
- MPX Group 1 STEC Amplification Tubes
- MPX Group 2 STEC Amplification Tubes
- Top 6 STEC Concentration Reagent
- Resuspension Buffer Tq
- Top STEC Wash Solution
Compatibility
BioControl GDS
Testing Method
PCR, Immunomagnetic Separation (IMS)
Legal Information
AOAC is a registered trademark of AOAC International
Assurance is a registered trademark of Merck KGaA, Darmstadt, Germany
Assurance GDS is a registered trademark of BioControl Systems, Inc.
PICKPEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Rotor-Gene is a registered trademark of Qiagen GmbH
mEHEC is a registered trademark of Merck KGaA, Darmstadt, Germany
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Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
wgk_germany
WGK 2
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