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S9430

Sigma-Aldrich

SYBR® Green I nucleic acid gel stain

greener alternative

10,000 × in DMSO

Synonym(s):

DNA stain, SYBR® green gel dye, safer gel stain

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About This Item

CAS Number:
163795-75-3
MDL number:
MFCD00284715
UNSPSC Code:
12171500
NACRES:
NA.52

form

solution

Quality Level

200

usage

 mL sufficient for 100 mini-gels

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

concentration

10,000 × in DMSO

technique(s)

PCR: suitable

greener alternative category

Aligned,

storage temp.

−20°C

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General description

SYBR® Green I is a proprietary asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system. SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm.

Application

SYBR® Green I nucleic acid gel stain has been used for:
  • the quantification of dsDNA
  • to stain DNA in polymerase chain reaction (PCR)
  • for comet assay technique
  • to assess spermatozoon membrane integrity
  • for visual inspection of DNA amplified by loop-mediated isothermal amplification (LAMP)
  • as a fluorescent dye in flow cytometry
  • real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for staining reverse-transcribed cDNA

Features and Benefits

  • An ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels
  • It can also detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any pre-washing steps
  • It is less mutagenic than ethidium bromide in Ames tests
  • It provides 50-100 times greater detection sensitivity than ethidium bromide for oligonucleotides
  • Useful for many applications with a limited amount of DNA
  • The binding of SYBR® Green I to DNA does not inhibit the activity of many common restriction endonucleases, including Hind III and EcoR I
  • Removal of this stain in-gel digestion and ligation techniques is not needed
  • SYBR Green I is a greener alternative product to ethidium bromide for staining

Storage and Stability

Store the product at –20 °C. The diluted Staining Solution may be stored, protected from light, either at 2–8 °C for several weeks or at room temperature for 3–4 days.

Legal Information

SYBR is a registered trademark of Life Technologies

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

201.2 °F - closed cup

flash_point_c

94 °C - closed cup

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Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications
Zipper H, et al.
Nucleic Acids Research, 32(12), e103-e103 (2004)
P A Morin et al.
BioTechniques, 19(2), 223-228 (1995-08-01)
A method for resolving and visualizing genotypes for simple sequence repeat loci on short (20 cm) polyacrylamide gels is described. This method makes use of a modified vertical electrophoresis cell to allow rapid electrophoresis without variation in migration rates due
Shear-resistant hydrogels to control permeability of porous tubular scaffolds in vascular tissue engineering
Tresoldi C, et al.
Materials Science and Engineering, C, 105, 110035-110035 (2019)
The in vitro effect of nonylphenol, propranolol, and diethylstilbestrol on quality parameters and oxidative stress in sterlet (Acipenser ruthenus) spermatozoa
Shaliutina O, et al.
Toxicology in vitro, 43, 9-15 (2017)
Loop mediated isothermal amplification of 5.8S rDNA for specific
detection of Tritrichomonas foetus
Jorge Oyhenart
Veterinary Parasitology, 193 (2013)
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