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B0904

Sigma-Aldrich

Basic Fuchsin

special for flagella, certified

Synonym(s):

Basic Red 9, Fuchsin basic, Magenta O, Pararosaniline

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About This Item

UNSPSC Code:
12171500
PubChem Substance ID:
24891537
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

200

type

special for flagella, certified

form

powder

composition

pararosaniline acetate, 3 parts
pararosaniline chloride, 1 part

color

green to very dark green

pH

5.0-6.0 (1 g/L)

mp

250 °C

solubility

H2O: 1 mg/mL

density

1.220 g/cm3

λmax

544 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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General description

Basic fuchsin, a triphenylmethane dye is commonly a mixture of pararosaniline (C.I. 42500), rosaniline (C.I. 42510), magenta II and new fuchsine (CI 42520). Basic fuchsin is widely used in tissue-specific staining of biological and coloring several materials including orlon, cotton, leather, paper etc. It is also used to stain collagen, muscle and mitochondria.

Application

Basic Fuchsin has been used in several biological tests including:
  • Acid-fast staining with carbol-fuchsine
  • Periodic Acid-Schiff method
  • Feulgen technique for DNA staining
  • ENDO culture medium for distinguishing between the bacteria Escherichia coli and Aerobacter aerogenes
  • Leifson′s method for bacterial flagella staining.

Biochem/physiol Actions

Basic fuchsin possesses anesthetic, bactericidal (gram-positive), and fungicidal properties.

Suitability

Certified for use in Leifson′s flagella stain.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H350

Hazard Classifications

Carc. 1B

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P2 (EN 143) respirator cartridges

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Analysis and testing of biological stains--the Biological Stain Commission Procedures.
D P Penney et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 77(5-6), 237-275 (2003-02-05)

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