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B8806

Sigma-Aldrich

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester

1 mg/mL in DMSO

Synonym(s):

BCECF-AM solution, Spiro(isobenzofuran-1(3H),9′-(9H)xanthene)-2′,7′-dipropanoic acid

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About This Item

EC Number:
UNSPSC Code:
12171500
NACRES:
NA.47

description

Mixture of six isomeric compounds

Quality Level

form

liquid

concentration

1 mg/mL in DMSO

color

colorless to yellow

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CC(=O)OCOC(=O)CCc1cc2c(Oc3cc(OCOC(C)=O)c(CCC(=O)OCOC(C)=O)cc3C24OC(=O)c5cc(ccc45)C(=O)OCOC(C)=O)cc1OCOC(C)=O

Application

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester has been used for the estimation of vacuolar pH and intracellular pH.

Biochem/physiol Actions

An intracellular fluorescent pH indicator.
Hydrolyzed to BCECF by cytosolic esterases.

Physical form

Solution in 0.1% BCECF AM Ester and 99.9% DMSO

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

185.0 °F

flash_point_c

85 °C


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