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D2935

Millipore

Decarboxylase broth base, Moeller

suitable for microbiology, NutriSelect® Plus

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

Quality Level

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | specific enzyme detection: suitable
microbiological culture: suitable

final pH

6.0±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages

microbiology

suitability

nonselective and differential for Citrobacter spp.
nonselective and differential for Enterobacter spp.
nonselective and differential for Escherichia coli
nonselective and differential for Klebsiella spp.
nonselective and differential for Proteus spp.
nonselective and differential for Salmonella spp.
nonselective and differential for Serratia spp.
nonselective and differential for Shigella spp.
nonselective and differential for coliforms
nonselective and differential for enterobacteriaceae

Application

Used to differentiate bacteria on the basis of their ability to decarboxylate amino acids.

Components

Ingredients (g/L)
Peptic digest of animal tissue, 5.00
Beef extract, 5.00
Dextrose, 0.50
Bromocresol purple, 0.01
Cresol red, 0.005
Pyridoxal, 0.005

Preparation Note

Suspend 10.52 g of Decarboxylase Broth Base in 1000 ml of distilled water. Add 10 g of either L-Arginine (Cat. No. 11010), or L-Lysine (Cat. No. 62840), or L-Ornithine (Cat. No. 75470). Heat to dissolve the medium completely. When L-ornithine is added, readjust the pH. Dispense in 5 ml quantities in screwcapped tubes. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 10 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Articles

Salmonella contamination is the second leading cause of food-borne illness worldwide. Controlling outbreaks of Salmonella is an important task for food regulators, restaurants and the food industry in general. The Salmonella family includes over 2,300 serotypes of bacteria, but two types, Salmonella enteritidis and Salmonella typhimurium, are responsible for about half of all human infections. Most outbreaks of Salmonella are traced back to dairy, poultry and meat products, but Salmonella can grow on nearly any food. Chicken, eggs and their derivative products are particularly high risk.

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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