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D7008

Sigma-Aldrich

Dihydroethidium

≥95% purity, powder

Synonym(s):

2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine, Hydroethidine

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About This Item

Empirical Formula (Hill Notation):
C21H21N3
CAS Number:
104821-25-2
Molecular Weight:
315.41
Beilstein/REAXYS Number:
5107482
MDL number:
MFCD00077335
UNSPSC Code:
12171500
PubChem Substance ID:
24894069
NACRES:
NA.47

product name

Dihydroethidium, ≥95%

Quality Level

100

assay

≥95%

form

powder

color

pink to very dark red

solubility

chloroform: 20 mg/mL

fluorescence

λex 370 nm; λem 420 nm (for cytoplasm of living cells)
λex 535 nm; λem 610 nm (for chromatin of living cells)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CCN1C(c2ccccc2)c3cc(N)ccc3-c4ccc(N)cc14

InChI

1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3

InChI key

XYJODUBPWNZLML-UHFFFAOYSA-N

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Related Categories

Application

Redox indicator. Blue fluorescence until oxidized to ethidium.
Dihydroethidium has been used as a superoxide probe in bovine pulmonary artery endothelial cell (BPAEC). It has been used to check the in situ generation of ROS (reactive oxygen species).

Packaging

Packaged under Argon.

Linkage

B-ring reduced analog of ethidium

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