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D9564

Sigma-Aldrich

DAPI, dilactate

≥98% purity (HPLC), powder

Synonym(s):

4′,6-Diamidino-2-phenyindole, dilactate

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About This Item

Empirical Formula (Hill Notation):
C16H15N5 · 2C3H6O3
Molecular Weight:
457.48
UNSPSC Code:
12171500
PubChem Substance ID:
329798905
NACRES:
NA.47

product name

DAPI, dilactate, ≥98% (HPLC)

Quality Level

300

assay

≥98% (HPLC)

form

powder

color

yellow

solubility

H2O: 20 mg/mL

ε (extinction coefficient)

28.00-32.50 at 345-351 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

CC(O)C(O)=O.CC(O)C(O)=O.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

InChI=1S/C16H15N5.C3H6O3/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;1-2(4)3(5)6/h1-8,21H,(H3,17,18)(H3,19,20);2,4H,1H3,(H,5,6)

InChI key

LCAUPANIXBMNIA-UHFFFAOYSA-N

Related Categories

General description

DAPI is an indolenine dye. It carries two amidine components, one on a pendant phenyl ring and another directly on the indole moiety.

Application

DAPI, dilactate has been used for the nuclear staining in cells. DAPI is a common flow cytometry stain that is widely used as a DNA and nuclear stain. Additionally, it is used as a counterstain following in situ hybridization and other immunofluorescence procedures.

Biochem/physiol Actions

A high sensitivity dye used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.

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