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M8010

Sigma-Aldrich

N,N′-Dimethyl-9,9′-biacridinium dinitrate

used as chemiluminescent reagent

Synonym(s):

9,9′-Bis(N-methylacridinium nitrate), Lucigenin

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About This Item

Empirical Formula (Hill Notation):
C28H22N4O6
CAS Number:
2315-97-1
Molecular Weight:
510.50
Beilstein/REAXYS Number:
3901768
EC Number:
219-023-4
MDL number:
MFCD00011925
UNSPSC Code:
12171500
PubChem Substance ID:
24897210
NACRES:
NA.47

Quality Level

100

form

powder

mp

245-250 °C

solubility

acetic acid: 10 mg/mL

fluorescence

λex 319 nm; λem 384 nm (pH 9.0)
λex 360 nm; λem 449 nm (Reaction product)
λex 368 nm; λem 505 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[O-][N+]([O-])=O.[O-][N+]([O-])=O.C[n+]1c2ccccc2c(-c3c4ccccc4[n+](C)c5ccccc35)c6ccccc16

InChI

1S/C28H22N2.2NO3/c1-29-23-15-7-3-11-19(23)27(20-12-4-8-16-24(20)29)28-21-13-5-9-17-25(21)30(2)26-18-10-6-14-22(26)28;2*2-1(3)4/h3-18H,1-2H3;;/q+2;2*-1

InChI key

KNJDBYZZKAZQNG-UHFFFAOYSA-N

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Application

N,N′-Dimethyl-9,9′-biacridinium dinitrate has been used in chemiluminescence assay to measure reactive oxygen species production and superoxide production.
Reagent used for the generation of chemiluminescence in the presence of a reductant and oxygen; it is employed in numerous chemiluminescent assays. Lucigenin is specific for superoxide anion radical.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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