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Canine MDR1 Knockout, Human BCRP/OATP2B1 double Knockin MDCKII Cells

canine kidney (cocker spaniel)

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

product name

Canine MDR1 Knockout, Human BCRP/OATP2B1 double Knockin MDCKII Cells,

biological source

canine kidney (cocker spaniel)

form

liquid

shipped in

dry ice

storage temp.

−196°C

Gene Information

human ... ABCG2(9429)

General description

MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous The canine MDR1 (cP-gp) efflux transporter gene has been effectively disrupted in both alleles. There is no expression of the cP-gp. Human BCRP and Human OATP2B1 has been transfected into the MDCKII cell line. Validation studies have shown BCRP dependent efflux of estrone-3 sulfate (requires OATP2B1) and teriflunomide (does not require OATP2B1).

Features and Benefits

MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm2. This strain is thought to be derived from the distal tubule or collecting duct of the nephron. The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells.

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Legal Information

ADME/Tox Cell Lines License and HepaRG Limited Use License

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Articles

CRISPR Engineered MDCKII Cells Without Canine P-glycoprotein

Madin-Darby Canine Kidney (MDCK) cells are one of the most widely used cell lines for a variety of research applications, including drug permeability and transporter studies.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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