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PHR1008

Supelco

Ascorbic Acid

Pharmaceutical Secondary Standard; Certified Reference Material

Synonym(s):

L-Ascorbic acid, L-Threoascorbic acid, Antiscorbutic factor, Vitamin C

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About This Item

Empirical Formula (Hill Notation):
C6H8O6
CAS Number:
50-81-7
Molecular Weight:
176.12
Beilstein/REAXYS Number:
84272
EC Number:
200-066-2
MDL number:
MFCD00064328
UNSPSC Code:
41116107
PubChem Substance ID:
329823039
E Number:
E300
NACRES:
NA.24

grade

certified reference material
pharmaceutical secondary standard

Quality Level

300

agency

traceable to BP 461
traceable to Ph. Eur. A1300000
traceable to USP 1043003

API family

ascorbic acid

form

solid

CofA

current certificate can be downloaded

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

mp

190-194 °C (dec.)

application(s)

food and beverages

format

neat

storage temp.

2-8°C

SMILES string

OC([C@]([C@@H](O)CO)([H])O1)=C(O)C1=O

InChI

1S/C6H8O6/c7-1-2(8)5-3(9)4(10)6(11)12-5/h2,5,7-10H,1H2/t2-,5+/m0/s1

InChI key

CIWBSHSKHKDKBQ-JLAZNSOCSA-N

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General description

Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards. Ascorbic acid is a powerful antioxidant and is widely used in pharmaceutical formulations. It has proven to have a beneficial effect on age-related diseases such as cancer, atherosclerosis, ocular, neurodegenerative diseases, etc. It is mainly involved in the formation and maintenance of collagen. The prolonged deficiency of ascorbic acid in diet may result in scurvy.

Application

Ascorbic Acid may be used as a pharmaceutical reference standard for the quantification of the analyte in pharmaceutical formulations using hydrophilic interaction chromatography.

Biochem/physiol Actions

Ascorbic Acid, also known as Vitamin C, is a six-carbon lactone produced by plants and some animal species but not by humans and other primates. Ascorbic acid functions as an enzymatic cofactor for multiple enzymes, serving as an electron donor for monooxygenases and dioxygenases. Ascorbic acid also functions as a powerful antioxidant, particularly in regards to reactive oxygen species.

Analysis Note

These secondary standards offer multi-traceability to the USP, EP (PhEur) and BP primary standards, where they are available.

Other Notes

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.

Footnote

To see an example of a Certificate of Analysis for this material enter LRAC2886 in the slot below. This is an example certificate only and may not be the lot that you receive.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
LRAD4554
LRAD2652
LRAD0386
LRAC2886
LRAB3631

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Simultaneous differential pulse voltammetric determination of ascorbic acid and caffeine in pharmaceutical formulations using a boron?doped diamond electrode
Lourencao CB, et al.
Electroanalysis, 22(15), 1717-1723 (2010)
L-ascorbic acid determination in pharmaceutical formulations using a biosensor based on carbon paste modified with crude extract of Zucchini (Cucurbita pepo)
Fatibello-Filho and Vieira CI
Journal of the Brazilian Chemical Society, 11(4), 412-418 (2000)
Method development and validation for optimised separation of salicylic, acetyl salicylic and ascorbic acid in pharmaceutical formulations by hydrophilic interaction chromatography and response surface methodology
Hatambeygi N, et al.
Journal of Chromatography A, 1218(35), 5995-6003 (2011)
Marc P Bradshaw et al.
Critical reviews in food science and nutrition, 51(6), 479-498 (2011-09-21)
Extensive reviews of research are available on the use of ascorbic acid, and its consequent degradation pathways, in physiological conditions or food matrices. However, very little information can be found for wine-related systems. This review highlights the relevant chemistry and
Noriko Umegaki-Arao et al.
Science translational medicine, 6(264), 264ra164-264ra164 (2014-11-28)
Revertant mosaicism is a naturally occurring phenomenon involving spontaneous correction of a pathogenic gene mutation in a somatic cell. It has been observed in several genetic diseases, including epidermolysis bullosa (EB), a group of inherited skin disorders characterized by blistering
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