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T4375

Sigma-Aldrich

Tetrazolium Blue Chloride

used in colorimetric determination of reducing compounds

Synonym(s):

3,3′-(3,3′-Dimethoxy[1,1′-biphenyl]-4,4′-diyl)-bis(2,5-diphenyl-2H-tetrazolium) dichloride, 3,3′-Dianisole-4,4′-bis(3,5-diphenyltetrazolium chloride), BTC, Blue Tetrazolium chloride, TZ

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About This Item

Empirical Formula (Hill Notation):
C40H32Cl2N8O2
CAS Number:
1871-22-3
Molecular Weight:
727.64
Beilstein/REAXYS Number:
3894077
EC Number:
217-488-8
MDL number:
MFCD00040933
UNSPSC Code:
12171500
PubChem Substance ID:
24900207
NACRES:
NA.47

Quality Level

200

form

powder

mp

255 °C (dec.) (lit.)

solubility

methanol: 50 mg/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccccc3)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccccc7)-c8ccccc8

InChI

1S/C40H32N8O2.2ClH/c1-49-37-27-31(23-25-35(37)47-43-39(29-15-7-3-8-16-29)41-45(47)33-19-11-5-12-20-33)32-24-26-36(38(28-32)50-2)48-44-40(30-17-9-4-10-18-30)42-46(48)34-21-13-6-14-22-34;;/h3-28H,1-2H3;2*1H/q+2;;/p-2

InChI key

MUUHXGOJWVMBDY-UHFFFAOYSA-L

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Application

Tetrazolium blue chloride (BTC) is a colorless tetrazolium compound that upon reduction forms a colored diformazan. The reactivity has been applied to the analysis of reducing compounds including the nanomolar detection of reducing sugars and carbidopa and methyldopa. BTC has been used for histological staining of mouse coronal brain sections and rat brain sections. In combination with 4-nitrocatechol, tetrazolium blue chloride forms a ternary complex with molybdenum(VI) for extraction and spectrophotometric determination of molybdenum. It has also been used to stain Francisella tularensis cells for tularemic microagglutination test.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H350

Hazard Classifications

Carc. 1B

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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2,3,5-Triphenyl-tetrazolium chloride solution suitable for microbiology, Filter sterilized solution that is recommended for the detection of microbial growth based on reduction of TTC

Millipore

17779

2,3,5-Triphenyl-tetrazolium chloride solution

[Development of a novel Francisella tularensis antigen stained with tetrazolium-blue for tularemia microagglutination test].
Celebi B
Mikrobiyoloji bulteni (2013)
Sensitive colorimetric assay of carbidopa and methyldopa using tetrazolium blue chloride in pharmaceutical products.
Issopoulos, P.B.
International Journal of Clinical Pharmacy, 11, 213-217 (1989)
Ternary complex of molybdenum(VI) with 4-nitrocatechol and tetrazolium blue chloride and Its application to extraction-spectrophotometric analysis of ferrous metallurgy products.
Dimitrov., A.N., et al.
Journal of Analytical Chemistry, 62, 122-125 (2007)
J J Li et al.
Neuroscience, 167(3), 665-677 (2010-03-09)
We reported previously that amoeboid microglial cells (AMC) in the developing brain exhibited endothelins (ETs) expression which diminished with advancing age and was undetected in microglia in the more mature brain. This study sought to explore if microglia in the
Preparation of a new tetrazolium salt which yields a blue pigment on reduction and its use in the demonstration of enzymes in normal and neoplastic tissues.
A M RUTENBURG et al.
Cancer research, 10(2), 113-121 (1950-02-01)
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