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Y0000664

Methotrexate impurity E

European Pharmacopoeia (EP) Reference Standard

Synonym(s):

4-{[(2,4-Diaminopteridin-6-yl)methyl]methylamino}benzoic acid

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About This Item

Empirical Formula (Hill Notation):
C15H14N7O2
CAS Number:
19741-14-1
Molecular Weight:
324.32
UNSPSC Code:
41116107
NACRES:
NA.24

grade

pharmaceutical primary standard

API family

methotrexate

manufacturer/tradename

EDQM

application(s)

pharmaceutical (small molecule)

format

neat

storage temp.

2-8°C

InChI

1S/C15H15N7O2/c1-22(10-4-2-8(3-5-10)14(23)24)7-9-6-18-13-11(19-9)12(16)20-15(17)21-13/h2-6H,7H2,1H3,(H,23,24)(H4,16,17,18,20,21)

InChI key

LWCXZSDKANNOAR-UHFFFAOYSA-N

Related Categories

General description

This product is provided as delivered and specified by the issuing Pharmacopoeia. All information provided in support of this product, including SDS and any product information leaflets have been developed and issued under the Authority of the Issuing Pharmacopoeia. For further information and support please go to the website of the issuing Pharmacopoeia.

Application

Methotrexate impurity E EP Reference standard, intended for use in laboratory tests only as specifically prescribed in the European Pharmacopoeia.

Packaging

The product is delivered as supplied by the issuing Pharmacopoeia. For the current unit quantity, please visit the EDQM reference substance catalogue.

Other Notes

Sales restrictions may apply.

related product

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Description
Pricing

M1000000

Methotrexate, European Pharmacopoeia (EP) Reference Standard

M1000008

Methotrexate impurity D, European Pharmacopoeia (EP) Reference Standard

Y0000602

Methotrexate for peak identification, European Pharmacopoeia (EP) Reference Standard

PHR1396

Methotrexate, Pharmaceutical Secondary Standard; Certified Reference Material

Y0001668

Methotrexate for system suitability, European Pharmacopoeia (EP) Reference Standard

1414003

Methotrexate, United States Pharmacopeia (USP) Reference Standard

1413988

R-Methotrexate, United States Pharmacopeia (USP) Reference Standard

1413999

Methotrexate System Suitability Mixture, United States Pharmacopeia (USP) Reference Standard

1414025

Methotrexate Related Compound B, United States Pharmacopeia (USP) Reference Standard

1414036

Methotrexate Related Compound C, United States Pharmacopeia (USP) Reference Standard

1414058

Methotrexate Related Compound E, United States Pharmacopeia (USP) Reference Standard

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Eye Irrit. 2 - Muta. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Folic acid ≥97%

Sigma-Aldrich

F7876

Folic acid

C Wolfrom et al.
European journal of clinical pharmacology, 39(4), 377-383 (1990-01-01)
The pharmacokinetics of methotrexate (MTX), 7-hydroxymethotrexate (7-OHMTX), 2,4-diaminomethylpteroic acid (APA), folinic acid, and 5-methyltetrahydrofolate (5-MTHF) have been studied during 21 high-dose MTX (HDMTX) infusions (5 g.m-2 in 24 h) with leucovorin (LCV) rescue, a component of the therapy of 5
H N Alkaysi et al.
Therapeutic drug monitoring, 12(2), 191-194 (1990-03-01)
A procedure for the determination of methotrexate in human plasma is reported. The analysis involved extraction of methotrexate as an ion pair in ethyl acetate. Reconstituted residue was analyzed using reverse phase C-18 column and a mobile phase consisting of
T E Ellenberger et al.
The Journal of biological chemistry, 264(27), 15960-15966 (1989-09-25)
We have examined the metabolism of the folate analog methotrexate (MTX) in the human parasite Leishmania major. These cells readily hydrolyzed MTX to N-10-methyl-4-deoxy-4-aminopteroate (MAPA), such that following a 24-h incubation in 1 microM [3H]MTX approximately 30% of the cell-associated
Rapid high-pressure liquid chromatographic determination of methotrexate and its metabolites 7-hydroxymethotrexate and 2,4-diamino-N(10)-methylpteroic acid in biological fluids.
H Breithaupt et al.
Analytical biochemistry, 121(1), 103-113 (1982-03-15)
Interference of 4-amino-4-deoxy-N10-methylpteroic acid with the radioimmunoassay of methotrexate.
J W Paxton
Clinical chemistry, 25(3), 491-492 (1979-03-01)
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