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04272

Sigma-Aldrich

Sodium phosphate dibasic dihydrate

meets analytical specification of Ph. Eur., BP, 98.5-101% (calc. to the dried substance)

Synonym(s):

sec-Sodium phosphate, Disodium hydrogen phosphate dihydrate, Disodium phosphate, di-Sodium hydrogen phosphate dihydrate

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About This Item

Linear Formula:
Na2HPO4 · 2H2O
CAS Number:
10028-24-7
Molecular Weight:
177.99
EC Number:
231-448-7
MDL number:
MFCD00149182
UNSPSC Code:
12161700
PubChem Substance ID:
329747906
NACRES:
NA.25

agency

meets analytical specifications of BP
meets analytical specifications of Ph. Eur.

Quality Level

200

assay

98.5-101% (calc. to the dried substance)

form

crystals

quality

meets analytical specification of Ph. Eur., BP

impurities

reducing matter, complies
 residual solvents, complies
≤0.001% heavy metals (as Pb)
≤1% NaH2PO4 (calc. to dried subst.)

loss

19.5-21% loss on drying, 130 °C

pH

9.0-9.4 (20 °C, 1%)

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤100 mg/kg

cation traces

As: ≤2 mg/kg
Fe: ≤10 mg/kg

suitability

complies for appearance of solution

SMILES string

O.O.[Na+].[Na+].OP([O-])([O-])=O

InChI

1S/2Na.H3O4P.2H2O/c;;1-5(2,3)4;;/h;;(H3,1,2,3,4);2*1H2/q2*+1;;;/p-2

InChI key

KDQPSPMLNJTZAL-UHFFFAOYSA-L

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Application

Sodium phosphate dibasic dihydrate has been used for isolating DNA and RNA from bacterial samples of human gastrointestinal (GI) tract.

Biochem/physiol Actions

Sodium phosphate dibasic dihydrate is an important component of running buffer of denaturing gel electrophoresis.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Erwin G Zoetendal et al.
Nature protocols, 1(2), 954-959 (2007-04-05)
The human gastrointestinal (GI) tract contains a complex microbial community that consists of numerous uncultured microbes. Therefore, nucleic-acid-based approaches have been introduced to study microbial diversity and activity, and these depend on the proper isolation of DNA, rRNA and mRNA.
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