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102-05F

Sigma-Aldrich

Human Epidermal Keratinocytes: HEK, fetal

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

human neonatal foreskin or adult skin (normal)

Quality Level

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

2n = 46

morphology

Epidermal

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

allergies; cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Epidermal keratinocytes comprise 90 % of all cells found in the epidermis, the outermost layer of skin. They produce keratin, the intermediate filament, largely responsible for skin’s barrier function. Through their interactions with epidermal melanocytes, keratinocytes obtain melanin and store it as a means of protection from UV radiation.

HEK provide an excellent model system to study many aspects of epithelial function and disease, particularly those related to skin biology and toxicology, as well as HPV infection.

When grown on inserts and provided with the liquid/air interface, HEK can differentiate into a stratified squamous epithelium and serve as a more physiological 3D tissue model for in vitro studies.

Cell Line Origin

Skin

Application

Skin biology and toxicology, wound healing, artificial skin, cell proliferation, migration, UV light response

Components

Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

  • Primary culture, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 16 doublings

Subculture Routine

Please refer to the HEK Culture Protocol.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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