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14360C

SAFC

EX-CELL® CD CHO Serum-Free Medium for CHO Cells, Chemically Defined

Animal-component free, without L-glutamine, without hypoxanthine, without thymidine, liquid, sterile-filtered, suitable for cell culture

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About This Item

MDL number:
MFCD01634638
UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

500

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

components

phenol red: yes
L-glutamine: no
NaHCO3: 1.6 g/L
glucose: 5.6 g/L
HEPES: 7.5 mM

shipped in

ambient

storage temp.

2-8°C

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Application

EX-CELL CD CHO is a chemically defined, animal-component free, serum-free medium developed for the long-term growth of Chinese Hamster Ovary (CHO) cells and expression of antibodies or protein products in suspension cell culture. CHO suspension cultures can be subcultured directly into EX-CELL CD CHO from serum-supplemented or serum-free medium with little or no adaptation. EX-CELL CD CHO is formulated without hypoxanthine, thymidine or L-glutamine, making it an appropriate medium for selection systems such as DHFR- and Glutamine Synthetase (GS System™). For applications that do not require the selective pressure of a hypoxanthine/thymidine (HT)-deficient medium, we recommend the use of EX-CELL CD CHO with hypoxanthine and thymidine (Catalog No. 14361C).

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.

Selection Process Development in a GS Knock-Out CHO Host Cell Line: The Effects of MSX Addition on Clones Generated in an MSX-Free Process

The Glutamine Synthetase (GS) expression system does not typically require multiple rounds of amplification to isolate high-producing clones (Brown, 1992).

Using Microarray Technology to Select Housekeeping Genes in Chinese Hamster Ovary Cells

In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

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