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EX-CELL® CD HEK293 Viral Vector Medium

Chemically defined, animal component-free, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Pharma Manufacturing

Synonym(s):

HEK293/HEK293T medium for viral vector production

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About This Item

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable (suspension)

components

L-glutamine: no

shipped in

wet ice

storage temp.

2-8°C

General description

EX-CELL CD HEK293 Viral Vector Medium is an animal origin-free, chemically defined cell culture medium for clinical and commercial manufacturing of viral vectors for gene and cell therapies. The medium is intended for processes implementing (polyethylenimine) PEI transfection reagents. EX-CELL CD HEK293 Viral Vector Medium is part of the VirusExpress® Lentiviral Production Platform and the medium of choice for VirusExpress 293T Lentiviral Production Cells (VP001). The medium is also suitable as a standalone solution for other HEK293 and HEK293T cell lines for AAV and lentiviral manufacturing. EX-CELL CD HEK293 Viral Vector Medium has been formulated to support high suspension cell densities with minimal clumping, high transient transfection efficiency when used with PEI, and high viral titers in shake flask and stirred tank bioreactor formats. Viral vector production using this medium is a scalable, suspension process that does not require a media exchange or feed post-transfection.

EX-CELL CD HEK293 Viral Vector Medium has been designed with long-term needs for manufacturing in mind, with a sustainable raw material supply chain, custom packaging options, and careful material and manufacturing process selection. The product is produced in an ISO9001-certified facility following the principles of cGMP according to IPEC-PQG.

Application

EX-CELL CD HEK293 Viral Vector Medium was developed to support VirusExpress Production Platforms. It performs well within the platform as well as with other HEK293 clones. The following white paper demonstrates the use of one of the prototype media formulations in developing a scalable, transient transfection-based suspension process for lentiviral vector production using the VirusExpress 293T Lentiviral Production cells:

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
VirusExpress is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Articles

A step-by-step overview of suspension-based, transient transfection bioreactor process development and scaleup of lentivirus production.

Scaling up viral vector production using adherent cell culture systems is challenging. Learn how suspension cell culture systems benefit large-scale bioprocessing.

To address scalability challenges of AAV manufacturing, we developed an HEK293 suspension cell line that can be used across many serotypes. Get the data in this article.

Related Content

A transfection-based solution to viral vector production using a suspension cell line, chemically defined medium, and a process with proven performance at scale.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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