18373
Atto 647N NHS ester
BioReagent, suitable for fluorescence, ≥90% (HPLC)
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product line
BioReagent
Quality Level
assay
≥90% (HPLC)
≥90% (degree of coupling)
form
solid
manufacturer/tradename
ATTO-TEC GmbH
λ
in ethanol (with 0.1% trifluoroacetic acid)
UV absorption
λ: 641-647 nm Amax
suitability
suitable for fluorescence
storage temp.
−20°C
Related Categories
General description
Atto 647N is a superior red-emitting fluorescence dye with a strong absorption, excellent fluorescence quantum yield (65%), high photostability, excellent ozone resistance, good solubility, and very little triplet formation.
Application
Atto 647N belongs to a new generation of fluorescent labels for the red spectral region. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, excellent fluorescence quantum yield, high photostability, excellent ozone resistance, good solubility, and very little triplet formation. Atto 647N is a cationic dye. The active ester of Atto 647N fluorescence dye reacts with amino groups under mild conditions. After coupling to a substrate the dye carries a net electrical charge of +1. In common with most Atto-labels, absorption and fluorescence are independent of pH in the range of 2 to 11, used in typical applications. As supplied Atto 647N consists of a mixture of two isomers with practically identical absorption and fluorescence properties.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
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