18507
Atto 565 maleimide
BioReagent, suitable for fluorescence
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About This Item
Empirical Formula (Hill Notation):
C37H37ClN4O10
Molecular Weight:
733.16
MDL number:
UNSPSC Code:
12352111
NACRES:
NA.32
Recommended Products
product line
BioReagent
Quality Level
assay
>90.0% (HPLC)
form
powder
manufacturer/tradename
ATTO-TEC GmbH
transmittance
254 nm
565 nm
fluorescence
λex 563 nm; λem 592 nm in 0.1 M phosphate pH 7.0
λ
(ethanol with 0.1% trifluoroacetic acid)
UV absorption
λ: 560-566 nm Amax
suitability
corresponds for coupling to thiols
suitable for fluorescence
storage temp.
−20°C
Application
Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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