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Sigma-Aldrich

4-Methylumbelliferyl butyrate

lipase substrate; esterase substrate, ≥95% (HPCE), powder or crystals, suitable for fluorescence

Synonym(s):

MU-Bu

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About This Item

Empirical Formula (Hill Notation):
C14H14O4
CAS Number:
17695-46-4
Molecular Weight:
246.26
Beilstein/REAXYS Number:
227973
EC Number:
241-694-7
MDL number:
MFCD00038087
UNSPSC Code:
12352204
PubChem Substance ID:
57647789
NACRES:
NA.32

product name

4-Methylumbelliferyl butyrate, suitable for fluorescence, ≥95% (HPCE)

Quality Level

100

assay

≥95% (HPCE)

form

powder or crystals

mp

90-92 °C (lit.)

solubility

DMF: soluble
DMSO: soluble

fluorescence

λex 312 nm in methanol
λex 360 nm; λem 449 nm (Reaction product)
λex 365 nm; λem 445 nm in 0.1 M Tris pH 8.0 (Lipase)

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CCCC(=O)Oc1ccc2C(C)=CC(=O)Oc2c1

InChI

1S/C14H14O4/c1-3-4-13(15)17-10-5-6-11-9(2)7-14(16)18-12(11)8-10/h5-8H,3-4H2,1-2H3

InChI key

WKPUJZVCZXWKCK-UHFFFAOYSA-N

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Application

suitable as fluorogenic substrate for esterases/lipases

Other Notes

Fluorogenic substrate for butyrate esterase

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Determination of lipase activity using image analysis.
Marcelo Müller-Santos et al.
Analytical biochemistry, 351(2), 305-307 (2006-03-10)
Ivan Kurtovic et al.
Fish physiology and biochemistry, 36(4), 1041-1060 (2010-02-10)
Lipases were purified from delipidated pyloric ceca powder of two New Zealand-sourced fish, Chinook salmon (Oncorhynchus tshawytscha) and hoki (Macruronus novaezelandiae), by fractional precipitation with polyethylene glycol 1000, followed by affinity chromatography using cholate-Affi-Gel 102, and gel filtration on Sephacryl
M Vaneechoutte et al.
Journal of clinical microbiology, 26(6), 1227-1228 (1988-06-01)
Branhamella catarrhalis can be distinguished from Neisseria spp. by the presence of butyrate esterase. This enzyme can be rapidly detected when 4-methylumbelliferyl butyrate is used as the substrate. All B. catarrhalis strains tested gave a positive fluorescence reaction within 5
Daniel Kübler et al.
European biophysics journal : EBJ, 44(8), 655-665 (2015-08-01)
Because of their vast diversity of substrate specificity and reaction conditions, lipases are versatile materials for biocatalysis. Lipase A from Bacillus subtilis (BSLA) is the smallest lipase yet discovered. It has the typical α/β hydrolase fold but lacks a lid
E Speeleveld et al.
Journal of clinical microbiology, 32(5), 1362-1363 (1994-05-01)
Moraxella catarrhalis can easily be differentiated from other oxidase-positive, gram-negative cocci with tributyrine, 4-methylumbelliferyl butyrate, or indoxyl acetate. All M. catarrhalis give positive reactions, and all Neisseria spp. give negative reactions. The 4-methylumbelliferyl butyrate tube test and indoxyl acetate strip
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