257R-1
GCDFP-15 (EP1582Y) Rabbit Monoclonal Primary Antibody
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
100
500
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
EP1582Y, monoclonal
description
For In Vitro Diagnostic Use in Select Regions (See Chart)
form
buffered aqueous solution
species reactivity
human
packaging
vial of 0.1 mL concentrate (257R-14)
vial of 0.5 mL concentrate (257R-15)
bottle of 1.0 mL predilute (257R-17)
vial of 1.0 mL concentrate (257R-16)
bottle of 7.0 mL predilute (257R-18)
manufacturer/tradename
Cell Marque™
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500
isotype
IgG
control
breast, breast carcinoma
shipped in
wet ice
storage temp.
2-8°C
visualization
cytoplasmic
Gene Information
human ... PIP(5304)
Related Categories
General description
Gross cystic disease fluid protein-15 (GCDFP-15) is one of four major component proteins found in the cystic fluid obtained from patients with fibrocystic changes of the breast. GCDFP-15 is a marker of apocrine glandular differentiation in both benign and malignant mammary epithelium. This protein has a widespread distribution in apocrine glands elsewhere in the axillary and perianal tissues, as well as in the sublingual and submaxillary salivary glands. The GCDFP-15 protein is a 15 kDa glycoprotein shown to be prolactin-inducible, and the GCDFP-15 gene has been cloned. Ultrastructurally, the GCDFP-15 protein has been localized in Golgi vesicle and cytoplasmic granules. The protein is released by exocytosis at the apices of the mammary epithelial cells. The primary application for this antibody product is the identification of breast carcinoma in the metastatic setting, or fluid analysis. In a study by Wick et al. on paraffin sections of 105 breast cancers and 585 non-mammary malignancies in order to assess its value in this context, anti-GCDFP-15 was compared with another putative mammary epithelial marker, alpha-lactalbumin (ALA), with respect to sensitivity and specificity for a diagnosis of breast carcinoma. Overall, the rates of specificity and sensitivity and positive predictive value for GCDFP-15 were 95%, 74%, and 74%, respectively. A consistent congruency between the reactivity patterns of primary and metastatic breast cancers was noted for GCDFP-15. Besides mammary carcinomas, the major tumor types that express GCDFP-15 were carcinomas of the salivary glands and sweat glands. Since the latter two types of lesions are unlikely to be diagnosed as metastatic breast cancer, statistical indices were recalculated after exclusion of these three tumor types. Following this exclusion, the adjusted rate of specificity of GCDFP-15 and positive predictive value for a diagnosis of metastatic carcinoma of the breast were each 99%. Another study by Bhargava et al. has shown GCDFP-15 staining of a higher percentage of cancer cells in lobular carcinoma as compared to ductal carcinoma of the breast.
Linkage
GCDFP-15 Positive Control Slides, Product No. 257S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).
Physical form
Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide
Preparation Note
Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.
Other Notes
For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com
Legal Information
Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany
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wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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S Ansai et al.
The American Journal of dermatopathology, 17(3), 249-255 (1995-06-01)
We investigated immunohistochemically the localization of lysozyme and Leu M1 in normal skin, 76 cases of benign sweat gland tumors, 28 cases of malignant sweat gland tumors, 23 cases of extramammary Paget's disease, 7 cases of sebaceous carcinoma, 6 cases
G Mazoujian et al.
The American journal of pathology, 110(2), 105-112 (1983-02-01)
Gross cystic disease fluid is a pathologic secretion from breast composed of several glycoproteins, including a unique 15,000-dalton monomer protein, GCDFP-15. By the immunoperoxidase technique, GCDFP-15 was localized in the apocrine metaplastic epithelium lining breast cysts and in apocrine glands
U Raju et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 6(5), 516-520 (1993-09-01)
Signet ring carcinoma of the breast often metastasizes to gastrointestinal tract and female genital tract. We report clinicopathologic features of 10 breast carcinomas with signet ring features, five of which had unusual metastatic patterns. The primary breast tumor in all
G Mazoujian et al.
The American Journal of dermatopathology, 10(1), 28-35 (1988-02-01)
Sixty-five cases of benign sweat gland tumors of the skin were studied for the expression and localization of gross cystic disease fluid protein-15 (GCDFP-15) by immunoperoxidase methods. There was positive staining of tumors of probable apocrine differentiation in 10 of
M R Wick et al.
Human pathology, 20(3), 281-287 (1989-03-01)
The identification of metastatic carcinoma of the breast may be difficult in the absence of a previous history of breast cancer. Various immunophenotypic markers have been introduced to aid in this process. A monoclonal antibody directed at a 15-kilodalton (kd)
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