65497
TRIS acetate-EDTA Buffer solution
for Northern and Southern blotting, solution
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About This Item
UNSPSC Code:
12161700
Recommended Products
grade
for molecular biology
product line
BioReagent
form
solution
impurities
DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected
pH
8.3±0.2
density
1.00 g/mL at 20 °C
storage temp.
room temp
General description
TBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but dsDNA runs faster with TAE buffer. This concentrate can be easily diluted to 0.5x before use (with molecular biology grade water).
Application
Suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. Especially useful for dsDNA separations.
Components
The TAE liquid buffer is a 1x solution that contains 0.04M TRIS acetate and 0.001M EDTA in water.
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