93350
Trizma® base
≥99.7% (T), crystalline, puriss p.a., aminopeptidase substrate
Synonym(s):
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
About This Item
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product name
Trizma® base, puriss. p.a., ≥99.7% (T)
description
aminopeptidase substrate
Quality Level
grade
puriss. p.a.
assay
≥99.7% (T)
form
crystalline
ign. residue
≤0.01% (as SO4)
pH
10.5-11.5 (25 °C, 10% in H2O)
useful pH range
7-9
pKa (25 °C)
8.1
bp
219-220 °C/10 mmHg (lit.)
mp
167-172 °C (lit.)
168-172 °C
solubility
water: soluble (678 g/l at 20 °C)
anion traces
chloride (Cl-): ≤5 mg/kg
sulfate (SO42-): ≤5 mg/kg
cation traces
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg
λ
10 % in H2O
UV absorption
λ: 230 nm Amax: ≤0.6
λ: 260 nm Amax: ≤0.05
λ: 280 nm Amax: ≤0.04
λ: 430 nm Amax: ≤0.01
SMILES string
NC(CO)(CO)CO
InChI
1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key
LENZDBCJOHFCAS-UHFFFAOYSA-N
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Related Categories
General description
Application
- In the preparation of TAE buffer for TAE agarose gel used to run PCR products by gel electrophoresis
- As a component of sample buffer for SDS-PAGE
- In the preparation of IEC (ion-exchange chromatography) buffer
Other Notes
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
related product
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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