Skip to Content
MilliporeSigma
All Photos(1)

Documents

93482

Sigma-Aldrich

Phenylmethanesulfonyl fluoride solution

~0.1 M in ethanol (T)

Synonym(s):

Benzylsulfonyl fluoride, PMSF

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
329-98-6
Beilstein/REAXYS Number:
2088311
MDL number:
MFCD00007424
UNSPSC Code:
12352202
PubChem Substance ID:
57653563
NACRES:
NA.77

biological source

microbial
synthetic

Quality Level

200

form

liquid

concentration

~0.1 M in ethanol (T)

density

0.797 g/mL at 20 °C

storage temp.

2-8°C

SMILES string

FS(=O)(=O)Cc1ccccc1

InChI

1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2

InChI key

YBYRMVIVWMBXKQ-UHFFFAOYSA-N

Application

Phenylmethanesulfonyl fluoride solution has been used as a component in the cell lysate buffer in breast adenocarcinoma cell line, fibrosarcoma cell line and D5 hindbrain and spinal cord neural progenitors.

Biochem/physiol Actions

Administration of PMSF produces analgesia unrelated to its anticholinesterase effect, and prolongs the analagesic effect of centrally administered β-endorphin.
Phenylmethanesulfonyl fluoride (PMSF) is a serine protease inhibitor. It binds covalently to active site residue of proteases and prevents proteolytic activity. PMSF is used in cell lysate buffers during protein purification to prevent target protein degradation. PMSF effectively inhibits the naloxone-precipitated withdrawal jumping.

pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2

wgk_germany

WGK 2

flash_point_f

closed cup

flash_point_c

closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 7

1 of 7

Leupeptin microbial, ≥90% (HPLC)

Sigma-Aldrich

L2884

Leupeptin

Sigma-Aldrich

Sigma-Aldrich

P2714

Protease Inhibitor Cocktail powder

Aprotinin from bovine lung saline solution, 3-7 TIU/mg protein

Sigma-Aldrich

A6279

Aprotinin from bovine lung

Sodium fluoride 0.5 M solution

Sigma-Aldrich

67414

Sodium fluoride 0.5 M solution

Phosphatase Inhibitor Cocktail 2 aqueous solution (dark coloration may develop upon storage, which does not affect the activity)

Sigma-Aldrich

P5726

Phosphatase Inhibitor Cocktail 2

Sodium Fluoride Solution

Sigma-Aldrich

919

Sodium Fluoride Solution

Sigma-Aldrich

Sigma-Aldrich

P8465

Protease Inhibitor Cocktail powder

SREBP1 drives KRT80-dependent cytoskeletal changes and invasive behavior in endocrine resistant ERalpha breast cancer
Magnani L, et al.
bioRxiv, 380634-380634 (2018)
Nervous system regionalization entails axial allocation before neural differentiation
Metzis V, et al.
Cell, 175(4), 1105-1118 (2018)
DNA-encoded library-derived DDR1 inhibitor prevents fibrosis and renal function loss in a genetic mouse model of Alport syndrome
Richter H, et al.
ACS Chemical Biology (2018)
Katrine Bugge et al.
Structure (London, England : 1993), 26(5), 734-746 (2018-04-17)
Communication within cells relies on a few protein nodes called hubs, which organize vast interactomes with many partners. Frequently, hub proteins are intrinsically disordered conferring multi-specificity and dynamic communication. Conversely, folded hub proteins may organize networks using disordered partners. In
C Pinsky et al.
Life sciences, 31(12-13), 1193-1196 (1982-09-20)
Intraperitoneal (IP) injection of the serine proteinase inhibitor phenylmethylsulfonyl fluoride (PMSF) produced dose-dependent analgesia in Sprague-Dawley rats. AD50 was 2.9 +/- 1.4 (S.E.) mg kg-1, the analgesia was antagonized by naloxone but unaffected by atropine. PMSF significantly enhanced the analgesic
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service