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D3812

Sigma-Aldrich

DirectLoad™ 50 bp DNA Step Ladder

ready-to-use marker for DNA electrophoresis

Synonym(s):

DirectLoad™ 50 bp DNA Marker, Ready-to-use 50 bp DNA Marker, Ready-to-use DirectLoad™ 50 bp DNA Marker, Ready-to-use DirectLoad™ 50 bp DNA Step Ladder

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About This Item

UNSPSC Code:
41105335
NACRES:
NA.25

Quality Level

200

form

(liquid)

usage

sufficient for 100 loads

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

Related Categories

General description

Sigma′s DirectLoad™ 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Application

DirectLoad™ 50 bp DNA Step Ladder has been used for cell lysis and genomic cleavage detection assay.
Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Features and Benefits

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Components

Sigma′s DirectLoad™ 50 bp DNA Step Ladder is provided in a gel-loading solution of 5% glycerol, 4.2 mM EDTA, 0.083% orange G and 0.0125% xylene cyanol.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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