LIG2
QuickLink™ DNA Ligation Kit
joins blunt-end and sticky-end DNA fragments
About This Item
Recommended Products
grade
for molecular biology
Quality Level
form
liquid
usage
kit sufficient for 50 ligation reactions
technique(s)
molecular cloning: suitable
shipped in
dry ice
storage temp.
−70°C
Related Categories
General description
Application
- Joining blunt or cohesive-end fragments of DNA into a cloning vector
- Recircularization of linear DNA
- Formation of concatamers
- dsDNA nick repair
Features and Benefits
- Fast 5 minutes ligation
- High ligation efficiency
- Room temperature reactions – no cooling required
- Perform bacterial transformation with the reaction mixture
Components
- 500uL 2X Ligation Buffer A (L9537)
- 100uL 5X Ligation Buffer B (L9662)
- 250 units T4 DNA Ligase (D2886) in 50% glycerol with 10 mM Tris-HCl (pH 7.5) 50 mM KCl, and 1 mM DTT
Principle
Legal Information
related product
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Protocols
T4 DNA ligase is used for the joining of DNA molecules with compatible cohesive (sticky) termini, joining of blunt ended double stranded DNA molecules
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