P6222
Precast Agarose Gels
8-well (portrait orientation)
Synonym(s):
8-well Precast Agarose Gels, Precast agarose gels for nucleic acid separation
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About This Item
UNSPSC Code:
41105300
NACRES:
NB.22
Recommended Products
grade
for molecular biology
Quality Level
form
gel form
does not contain
ethidium bromide
concentration
1.25% agarose (in 1x MOPS buffer; for RNA electrophoresis)
storage temp.
room temp
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General description
Precast agarose gels are designed for high resolution separation of nucleic acids, producing sharp RNA bands with low background fluorescence. Precast gels are individually foil sealed in plastic trays and are supplied in a box of twenty.
Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Cassette size: 6.8 × 10.2 cm container tray (fits common horizontal electrophoresis units)
Well orientation: 8-well, portrait style
Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Cassette size: 6.8 × 10.2 cm container tray (fits common horizontal electrophoresis units)
Well orientation: 8-well, portrait style
Application
Precast Agarose Gels has been used for the electrophoresis of purified PCR products.
Precast agarose gels are designed for high resolution separation of nucleic acids, producing sharp DNA and RNA bands with low background fluorescence. Precast gels are individually foil sealed in plastic trays that help eliminate contamination from equipment and handling. Gels can be electrophoresed right in the plastic tray.
TBE - agarose gels are available in two common concentrations (1% and 4%) for DNA electrophoresis. Ethidium bromide (0.5 μg/ml) is included in the TBE-agarose gels for easy visualization. TBE-agarose gels are available in 8, 20 and 24 well formats. The separation range for the 1.0% gel is 500-1000 bp and 8-1000 bp for the 4% gel.
MOPS - agarose gels (1.25%) are available without denaturants (e.g. formaldehyde or glyoxal) for the analysis of total RNA, in vitro RNA transcripts, and Northern blotting. RNA electrophoresis usually does not interfere with staining. If significant secondary structure is suspected, the RNA should be denatured by a method listed in the product insert. The separation range for the 1.25% MOPS gel is 0.25-10 kb.
TBE - agarose gels are available in two common concentrations (1% and 4%) for DNA electrophoresis. Ethidium bromide (0.5 μg/ml) is included in the TBE-agarose gels for easy visualization. TBE-agarose gels are available in 8, 20 and 24 well formats. The separation range for the 1.0% gel is 500-1000 bp and 8-1000 bp for the 4% gel.
MOPS - agarose gels (1.25%) are available without denaturants (e.g. formaldehyde or glyoxal) for the analysis of total RNA, in vitro RNA transcripts, and Northern blotting. RNA electrophoresis usually does not interfere with staining. If significant secondary structure is suspected, the RNA should be denatured by a method listed in the product insert. The separation range for the 1.25% MOPS gel is 0.25-10 kb.
Packaging
Package of 20 individually foil-wrapped gels.
Other Notes
DNA agarose gels contain 1× TBE buffer (0.089 M Tris-borate, pH 8.3, 2 mM EDTA) and 0.5 mg/ml ethidium bromide.
RNA agarose gels contain 1× MOPS (20 mM MOPS, 5 mM sodium acetate, 1 mM EDTA and 1 mM EGTA, pH 7.0)
RNA agarose gels contain 1× MOPS (20 mM MOPS, 5 mM sodium acetate, 1 mM EDTA and 1 mM EGTA, pH 7.0)
Quality
No detected DNase or RNase activity
Physical form
Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Cassette size: 6.8 × 10.2 cm container tray; fits common horizontal electrophoresis units
Sample format: 8-well (portrait), 20-well (landscape), 24-well (2 × 12 well portrait)
Cassette size: 6.8 × 10.2 cm container tray; fits common horizontal electrophoresis units
Sample format: 8-well (portrait), 20-well (landscape), 24-well (2 × 12 well portrait)
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