R4142
RNA Marker template set
~0.5 mg/mL in 10 mM Tris-HCl, pH 7.5, 1 mM EDTA
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About This Item
UNSPSC Code:
12352200
grade
for molecular biology
concentration
~0.5 mg/mL in 10 mM Tris-HCl, pH 7.5, 1 mM EDTA
storage temp.
−20°C
Application
In vitro transcription with T7 RNA polymerase produces transcripts 100, 200, 300, 400, 600, 800 and 1,000 bases long. The transcripts can be labeled by including biotinylated, radioactive, or other labeled rNTP′s in the reaction. The markers are useful for RNase protection assays or for electrophoresis of low molecular weight RNA fragments.
Other Notes
A mixture of seven linearized plasmids for use as templates for RNA markers.
Certificates of Analysis (COA)
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