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SEQXE

Sigma-Aldrich

SeqPlex DNA Amplification Kit

For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.

Synonym(s):

SeqPlex Enhanced DNA Amplification Kit, WGA kit, whole genome amplification kit

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.55

Quality Level

200

technique(s)

whole genome amplification: suitable

shipped in

wet ice

storage temp.

−20°C

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General description

SeqPlex™ Enhanced DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA. The yields from chromatin immunoprecipitation (ChIP) or formalin-fixed paraffin-embedded tissue samples (FFPE) are often less than required for successful next generation sequencing library preparation. The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented DNA samples for input into a NGS workflow. This kit is an extension of the WGA product line and has been developed to integrate into the Illumina®, SOLiD™, or 454 sequencing workflows.

Application

SeqPlex™ DNA Amplification Kit has been used for whole genome amplification.

Features and Benefits

  • Random priming technology amplifies fragmented DNA such as ChIP or FFPE
  • Facilitates sequencing from as little as 100 pg of ChIP DNA
  • Enhanced primers for complete genome coverage, minimal sequence bias, primer removal, and amplicon size ideal for next gen sequencing
  • Compatible with Illumina®, SOLiD™, or 454 library prep for next generation sequencing

Other Notes

SEQXE-500RXN is manufactured on-demand. Contact technical services at techserv@sial.com for more information.

Legal Information

Illumina is a registered trademark of Illumina, Inc.
SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
iCAT is a registered trademark of University of Washington

Kit Components Also Available Separately

Product No.
Description
SDS
  • W4502Water, Nuclease-Free Water, for Molecular BiologySDS
  • Library Preparation Buffer

recommended

Product No.
Description
Pricing

required but not provided

Product No.
Description
Pricing

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Protocols

SeqPlex Enhanced DNA Amplification Kit (SEQXE) Protocol

The SeqPlex DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA

Imprint® Chromatin Immunoprecipitation Kit (CHP1) Protocol

The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format

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