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T9285

Sigma-Aldrich

Tris-EDTA buffer solution

100X concentrate for Northern and Southern blotting

Synonym(s):

Tris-EDTA wash buffer, TE buffer solution

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About This Item

MDL number:
MFCD00236359
UNSPSC Code:
41105319
PubChem Substance ID:
329826904
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

sterility

0.2 μm filtered

form

liquid

pH

7.8-8.2 (1 × in water)

application(s)

sample preparation

foreign activity

DNase, RNase, none detected

storage temp.

room temp

SMILES string

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

InChI key

VLEIUWBSEKKKFX-UHFFFAOYSA-N

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General description

Tris EDTA buffer is commonly used in molecular biology for reconstitution and storage of many precipitated biomolecules, including DNA and RNA. Tris-HCl is a commonly used buffer and EDTA is a metal chelating agent. TE buffer solubilizes nucleic acids while protecting the molecules from degradation. It is used in nucleic acid isolation, which may be done prior to nucleic acid gel electrophoresis and Northern or Southern blot hybridization.

Application

TE buffer has been used for:
  • isolate, resuspend, or store DNA and RNA for PCR or qPCR
  • gel electrophoresis, including Northern and Southern blotting
  • bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells
  • antigen retrieval for immunohistochemistry

Components

TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

Other Notes

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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