A0521
α-Amylase from human saliva
Type IX-A, lyophilized powder, 1,000-3,000 units/mg protein
Synonym(s):
1,4-α-D-Glucan-glucanohydrolase
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About This Item
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type
Type IX-A
Quality Level
form
lyophilized powder
specific activity
1,000-3,000 units/mg protein
composition
Protein, 5.0-15.0% biuret
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Waste Prevention
Design for Energy Efficiency
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UniProt accession no.
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storage temp.
−20°C
Gene Information
human ... AMY1A(276)
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General description
α-amylase is the main secretory protein constituent of saliva. It contains 496 amino acids and has two isoforms, glycosylated and non-glycosylated. α-amylase gene is located on human chromosome 1p21.1. α-amylase is mainly produced in pancreas and saliva.
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Application
α-amylase from human saliva has been used to test α-amylase inhibitors using HPLC (high performance liquid chromatography). It also has been used to study the digestibility of human milk oligosaccharides (HMO).
Biochem/physiol Actions
α-amylase catalyses the hydrolysis of α-1,4 glycosidic linkage in oligosaccharides. It plays a crucial role in the initial digestion of starch, glycogen and polysaccharides. Elevated levels of amylase in serum is linked to acute pancreatitis.
Unit Definition
One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.
Physical form
Lyophilized powder containing (NH4)2SO4 and sodium citrate
Preparation Note
Chromatographically purified
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
substrate
Certificates of Analysis (COA)
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