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A1418

Sigma-Aldrich

Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Mouse IgG (Fc specific)–AP

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About This Item

MDL number:
MFCD00162626
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

200

recombinant

expressed in goat

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

should not react with

human

technique(s)

direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25
western blot: 1:50,000-1:100,000 using total cell extract of HeLa cells

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

IgG antibody plays a crucial role in humoral immune responses such as complement activation, phagocytosis, placental transport and cell surface-receptor binding. Anti-mouse IgG (Fc specific)–alkaline phosphatase antibody (diluted 1: 10,000 in PBS-Tween) can be used in ELISA. It is also useful in immunoblotting. Goat anti-mouse IgG (Fc specific)–alkaline phosphatase antibody reacts specifically with mouse IgG Fc fragment, IgG and its subclasses IgG1, IgG2a, IgG2b and IgG3 but not with human IgG, and mouse IgA or IgM.

Immunogen

Purified mouse IgG Fc fragment.

Application

Alkaline phosphatase-conjugated goat anti-mouse Fc specific antibody was used as a secondary antibody in ELISA assays at a dilution of 1:1000 in PBS/0.1% Tween and 1% BSA for 1.5 hours at 37°C. Antibody was developed using 4-nitrophenyl phosphate (Sigma) as a substrate for 30 minutes at 37°C.
Anti-mouse IgG (Fc specific)–alkaline phosphatase antibody can be used in ELISPOT (immunospot) assay. It can also be used in immunohistochemistry and western blot.

Other Notes

Antibody adsorbed with human IgG.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide

Preparation Note

Adsorbed to reduce background with human samples.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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D J Lewis et al.
European journal of immunology, 21(9), 2087-2094 (1991-09-01)
The immune response to cholera toxin B subunit given orally was studied in 13 human volunteers. A serum IgG and IgA antitoxin response was observed, which was boosted by a second immunization. Using an immunospot assay, cells spontaneously secreting anti-toxin
Development of a Surface Plasmon Resonance?Based
Immunoassay for Listeria monocytogenes
Leonard P, et al.
Journal of Food Protection, 68(4), 728-735 null
Viswanath Arutla et al.
ACS combinatorial science, 19(5), 286-298 (2017-04-07)
Since the demonstration of nicotine vaccines as a possible therapeutic intervention for the effects of tobacco smoke, extensive effort has been made to enhance nicotine specific immunity. Linker modifications of nicotine haptens have been a focal point for improving the
G Kijanka et al.
Gut, 59(1), 69-78 (2009-10-16)
Patients with cancer have antibodies against tumour antigens. Characterising the antibody repertoire may provide insights into aberrant cellular mechanisms in cancer development, ultimately leading to novel diagnostic or therapeutic targets. The aim of this study was to characterise the antibody
Edward W Dervan et al.
Investigative ophthalmology & visual science, 51(6), 2968-2975 (2010-01-29)
Complex repertoires of IgG autoantibodies have been detected against ocular antigens in patients with glaucoma. The goal was to identify and characterize the IgG autoantibody repertoires in sera of patients with pseudoexfoliation glaucoma (PXFG) with protein macroarrays. Serum samples of
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