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A2278

Sigma-Aldrich

N-Acetyl-D-glucosamine–Agarose

saline suspension

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About This Item

MDL number:
MFCD00136044
UNSPSC Code:
41106500
PubChem Substance ID:
24890642
NACRES:
NA.56

form

saline suspension

Quality Level

200

matrix

6% beaded agarose

matrix activation

divinyl sulfone

matrix attachment

hydroxyl

matrix spacer

5 atoms

capacity

≥25 mg/mL binding capacity (Triticum vulgaris lectin)

storage temp.

2-8°C

SMILES string

CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO

InChI

1S/C8H15NO6/c1-4(12)9-5(2-10)7(14)8(15)6(13)3-11/h2,5-8,11,13-15H,3H2,1H3,(H,9,12)/t5-,6+,7+,8+/m0/s1

InChI key

MBLBDJOUHNCFQT-LXGUWJNJSA-N

General description

N-Acetyl-D-glucosamine is used in affinity chromatography, protein chromatography, and carbohydrate matrices. N-Acetyl-D-glucosamine can be used to purify wheat germ (Triticum vulgaris) lectin.

Application

N-Acetyl-D-glucosamine-Agarose has been used

  • for the incubation of supernatant for the depletion of ficolin-3-deficient serum
  • for detecting the lectin activity by ligand binding assays
  • to produce ficolin 2 depleted serum

Physical form

Suspension in 0.15 M NaCl, 0.01 M sodium phosphate, pH 6.8, containing 0.02% sodium azide

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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M B Allen et al.
The Biochemical journal, 185(3), 577-582 (1980-03-01)
1. Under normal assay conditions the N-acetyl-D-glucosamine kinases from rat liver and kidney show a pH-dependent lag phase before reaching a steady state, which is probably due to reversible dissociation of the dimeric enzyme. 2. The enzyme catalyses the phosphorylation
Comparative Biochemistry of Molecular Targets for Drug and Pesticides: N-acetyl-D-glucosamine and Acetylcholine Related Enzymes
Yang, Q.Y.
Current Drug Targets (2012)
M B Allen et al.
The Biochemical journal, 185(3), 565-575 (1980-03-01)
1. Procedures for the extensive purification in high yield of N-acetyl-D-glucosamine kinase from rat liver and kidney are described. The separation of this enzyme from hepatic glucokinase depended primarily on their differing behaviour on an affinity column of Sepharose--N-(6-aminohexanoyl)-2-amino-2-deoxy-D-glucopyranose. 2.
J B Pitner et al.
Carbohydrate research, 324(1), 17-29 (2000-03-21)
The binding of Strep 9, a mouse monoclonal antibody (mAb) of the IgG3 subclass directed against the cell-wall polysaccharide of Group A Streptococcus (GAS), has been characterized. The intact antibody and proteolytic fragments of Strep 9 bind differently to GAS:
R Sullivan et al.
Biochemical and biophysical research communications, 162(1), 184-188 (1989-07-14)
Human ejaculated spermatozoa are heterogeneous and can be separated into two distinct populations according to their respective buoyant densities. In order to investigate the functional differences between these two types of spermatozoa, we have searched for the presence of galactosyltransferase.
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