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A2792

Sigma-Aldrich

Acrylamide/Bis-acrylamide

BioReagent, for molecular biology, 29:1 (ratio)

Synonym(s):

Acrylamide/Bisacrylamide, 29:1 (3.3% crosslinker), Polyacrylamide solution

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About This Item

MDL number:
MFCD00080848
UNSPSC Code:
41105319
PubChem Substance ID:
329770795
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

product line

BioReagent

form

powder

feed ratio

29:1 (ratio)

foreign activity

DNase, RNase and protease, none detected

SMILES string

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

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General description

Acrylamide (AA) is a neurotoxin and carcinogen. It was found in potato products like, french fries and potato chips and also in several cereal-based products. This highly reactive organic compound has the ability to polymerize to form polyacrylamide.

Application

Acrylamide/Bis-acrylamide has been used in the preparation of polyacrylamide (PA) gels. It has also been used as a component in the preparation of blotting gel precursor solution, separation gel precursor solution and loading gel precursor solution.
Ready to reconstitute dry powder blends are accurately pre-blended to produce a 40% (w/v) stock solution for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Features and Benefits

Eliminates the need to weigh toxic acrylamide and bis-acrylamide.

Reconstitution

Reconstitute by slowly adding the indicated amount of distilled water to the container and dissolve contents.
To make up 100 ml of A2792, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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