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A5043

Sigma-Aldrich

Adenosine Deaminase from bovine spleen

Type X, buffered aqueous glycerol solution, ≥130 units/mg protein

Synonym(s):

ADA, adenosine aminohydrolase, Adenosine aminohydrolase

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About This Item

CAS Number:
9026-93-1
Enzyme Commission number:
3.5.4.4 (BRENDA, IUBMB)
MDL number:
MFCD00081287
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bovine spleen

Quality Level

200

type

Type X

form

buffered aqueous glycerol solution

specific activity

≥130 units/mg protein

mol wt

32.5-33 kDa

cation traces

NH4+: ≤10 μg/mg protein

UniProt accession no.

P56658

foreign activity

alkaline phosphatase ≤0.01%
guanase ≤0.01%
nucleoside phosphorylase ≤0.1%

storage temp.

2-8°C

Gene Information

cow ... ADA(280712)

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General description

Molecular weight: 32.5-33 kDa

pI: 4.85

Application

Adenosine Deaminase from bovine spleen has been used in the immobilization on biostrip for stability and catalytic studies.
Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Biochem/physiol Actions

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.

Unit Definition

One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.

Physical form

Solution in 50% glycerol, 5 mM potassium phosphate, pH 6.0

Analysis Note

Protein determined by biuret.

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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