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A6066

Sigma-Aldrich

Anti-Rat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Rabbit Anti-Rat IgG (whole molecule)–AP

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About This Item

MDL number:
MFCD00162809
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

technique(s)

direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100
western blot: 1:30,000-1:60,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

An immunoglobulin has two heavy chains and two lights chain connected by disulfide bond. It mainly helps in immune defense. IgG is a glycoprotein and is the major class of immunoglobulin. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c.
IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins.
The affinity isolated antibody is obtained from anti-rat IgG antiserum by immunospecific purification, which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to rat IgG. Anti-Rat IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde
Identity and purity of the antibody is established by immunoelectrophoresis (IEP) prior to conjugation. Electrophoresis of the product followed by diffusion versus anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation.

Specificity

Binds all rat Igs. Anti-Rat IgG (whole molecule)-Alkaline Phosphatase antibody binds to all rat immunoglobulins.

Immunogen

Purified rat IgG

Application

Anti-Rat IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit has been used in:
  • western blot analysis
  • indirect enzyme-linked immunosorbent assay (ELISA)
  • RNA immunoprecipitation assay

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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