A7030
Bovine Serum Albumin
heat shock fraction, protease free, fatty acid free, essentially globulin free, pH 7, ≥98%
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
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About This Item
Recommended Products
biological source
bovine
Quality Level
assay
≥98%
form
lyophilized powder
mol wt
~66 kDa
purified by
heat shock fractionation
origin
USA origin
free fatty acid content
≤0.02%
technique(s)
ELISA: suitable
blood typing: suitable
ligand binding assay: suitable
impurities
essentially globulin free
loss
≤5%
pH
7
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
protease ≤0.005 U/mg
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
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General description
Bovine serum albumin is a protein which has a key role in regulating blood colloidal osmotic pressure. A water-soluble protein composed of 583 amino acids, BSA has a calculated molecular weight of 66,430 Daltons. Six α-helices form three homologous domains of BSA. Depending on pH, BSA undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.
This BSA product is specifically evaluated and tested for the following properties:
Protease-free BSA is especially useful for applications like:
b. Nucleic acid hybridization
c. Radioimmunoassay (RIA)
This BSA product is specifically evaluated and tested for the following properties:
- Very low protease content / protease-free
- Very low fatty acid content / fatty acid-free
Protease-free BSA is especially useful for applications like:
- Enzyme assays
- Protein-based assays
- Protease-sensitive techniques that include:
b. Nucleic acid hybridization
c. Radioimmunoassay (RIA)
Application
Bovine Serum Albumin has been used:
This A7030 BSA has specifically been used as a component of blocking buffer in a peptide-based ELISA assay for analysis of COVID-19 / SARS-CoV-2 samples (Poh, C.K. et al., Nat. Commun., 11(1), 2806 (2020)).
- as a constituent of medium used for the differentiation of human pluripotent stem cells
- in enzyme-linked immunosorbent assay (ELISA) and sandwich ELISA
- to permeabilize fetal cortical cells
This A7030 BSA has specifically been used as a component of blocking buffer in a peptide-based ELISA assay for analysis of COVID-19 / SARS-CoV-2 samples (Poh, C.K. et al., Nat. Commun., 11(1), 2806 (2020)).
Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Features and Benefits
- Heat shock fractionated
- Protease-free
- Fatty acid-free
- Essentially globulin-free
Preparation Note
Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
also commonly purchased with this product
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
Certificates of Analysis (COA)
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