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A9434

Sigma-Aldrich

Ammonium chloride

for molecular biology, suitable for cell culture, ≥99.5%

Synonym(s):

Salmiac

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About This Item

Linear Formula:
NH4Cl
CAS Number:
12125-02-9
Molecular Weight:
53.49
Beilstein/REAXYS Number:
4371014
EC Number:
235-186-4
MDL number:
MFCD00011420
UNSPSC Code:
12352302
eCl@ss:
38050207
PubChem Substance ID:
24891452
NACRES:
NA.31

grade

for molecular biology

Quality Level

200

vapor density

1.9 (vs air)

vapor pressure

1 mmHg ( 160.4 °C)

assay

≥99.5%

form

powder or crystals

technique(s)

cell culture | mammalian: suitable

mp

340 °C (subl.) (lit.)

anion traces

phosphate (PO43-): ≤2 ppm
sulfate (SO42-): ≤20 ppm

cation traces

Fe: ≤2 ppm
heavy metals (as Pb): ≤5 ppm

foreign activity

DNase, RNase, protease, none detected

SMILES string

N.Cl

InChI

1S/ClH.H3N/h1H;1H3

InChI key

NLXLAEXVIDQMFP-UHFFFAOYSA-N

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Application

Ammonium chloride has been used:
  • as an inhibitor of endosome acidification in vero cells
  • as an autophagy inhibitor in vascular smooth muscle cell (VSMC)
  • in the depletion and lysis of erythrocytes in testis parenchyma homogenate

Biochem/physiol Actions

Ammonium chloride is one of the three principal components of the nitrogen cycle. Ammonium chloride administration promotes creatinine and urea clearance andinduces metabolic acidosis in mice. Excess of ammonium chloride inhibits Kreb′s pathway in brain and depletes ATP in astrocytes. Ammonium chloride inhibits acidification in the endosome-lysosome system. Ammonium chloride is a hemolysing agent used for the lysis of erythrocytes.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Infectious bronchitis virus entry mainly depends on clathrin mediated endocytosis and requires classical endosomal/lysosomal system
Wang H, et al.
Virology, 528, 118-136 (2019)
Effect of ammonium chloride and dietary phosphorus in the azotaemic rat. I. Renal function and biochemical changes
Jara A, et al.
Nephrology, Dialysis, and Transplantation, 19(8), 1986-1992 (2004)
Nrf2 Activation Provides Atheroprotection in Diabetic Mice Through Concerted Upregulation of Antioxidant, Anti-inflammatory, and Autophagy Mechanisms
Lazaro I, et al.
Frontiers in Pharmacology, 9, 118-136 (2018)
Aqueous ammonia and ammonium chloride hydrates: Principal infrared spectra
Max JJ and Chapados C
Journal of Molecular Structure, 1046, 124-135 (2013)
Induction of metabolic acidosis with ammonium chloride (NH4Cl) in mice and rats-species differences and technical considerations
Nowik M, et al.
Cellular Physiology and Biochemistry, 26(6), 1059-1072 (2010)
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Protocols

GenElute™ Mammalian Genomic DNA Miniprep Kit Protocol

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

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