Skip to Content
MilliporeSigma
All Photos(6)

Documents

A9528

Sigma-Aldrich

Amphotericin B solubilized

powder, suitable for cell culture, BioXtra

Synonym(s):

Amphotericin B from Streptomyces sp., Fungizone

Sign Into View Organizational & Contract Pricing
All Photos(6)

About This Item

Empirical Formula (Hill Notation):
C47H73NO17
CAS Number:
1397-89-3
Molecular Weight:
924.08
Beilstein/REAXYS Number:
78342
MDL number:
MFCD00877763
UNSPSC Code:
12352207
eCl@ss:
34010400
PubChem Substance ID:
24891468
NACRES:
NA.76

product name

Amphotericin B solubilized, powder, γ-irradiated, BioXtra, suitable for cell culture

Quality Level

300

sterility

γ-irradiated

product line

BioXtra

form

powder

technique(s)

cell culture | mammalian: suitable

antibiotic activity spectrum

fungi
yeast

mode of action

cell membrane | interferes

storage temp.

2-8°C

SMILES string

C[C@H]1O[C@@H](O[C@@H]2C[C@@H]3O[C@](O)(C[C@@H](O)C[C@@H](O)[C@H](O)CC[C@@H](O)C[C@@H](O)CC(=O)O[C@@H](C)[C@H](C)[C@H](O)[C@@H](C)\C=C\C=C\C=C\C=C\C=C\C=C\C=C\2)C[C@H](O)[C@H]3C(O)=O)[C@@H](O)[C@@H](N)[C@@H]1O

InChI

1S/C47H73NO17/c1-27-17-15-13-11-9-7-5-6-8-10-12-14-16-18-34(64-46-44(58)41(48)43(57)30(4)63-46)24-38-40(45(59)60)37(54)26-47(61,65-38)25-33(51)22-36(53)35(52)20-19-31(49)21-32(50)23-39(55)62-29(3)28(2)42(27)56/h5-18,27-38,40-44,46,49-54,56-58,61H,19-26,48H2,1-4H3,(H,59,60)/b6-5+,9-7+,10-8+,13-11+,14-12+,17-15+,18-16+/t27-,28-,29-,30+,31+,32+,33-,34-,35+,36+,37-,38-,40+,41-,42+,43+,44-,46-,47+/m0/s1

InChI key

APKFDSVGJQXUKY-INPOYWNPSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Chemical structure: polyene

Application

Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.

Biochem/physiol Actions

Amphotericin B binds to sterols, forming pores in the membrane, and causing small molecules to leak out. The product is most effective against fungi and yeast.

Packaging

Solubilized Amphotericin B is packaged in amber glass bottle, with the contents inside an inserted fused cone.

Components

This product is a colloidal suspension of Amphotericin B, using deoxycholate as the solubilizing agent. The formulation is ~45% Amphotericin B and 35% sodium deoxycholate in sodium phosphate and sodium chloride.

Caution

Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years.

Preparation Note

Amphotericin B is soluble at 19.6-20.4 mg/ml in water, at natural pH without heating, giving a yellowish clear to slightly hazy solution.
Aqueous solutions cannot be sterile filtered due to poor solubility. If reconstituted in sterile water, there is no need to filter sterilize.

It is also soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL).

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Product is sensitive to light and moisture. Keep in a dry place.
The actual solid weight of the packaged product is calculated according to the UV purity.

Kit Components Only

Product No.
Description
  • Amphotericin B ~ 45 %
  • Sodium deoxycholate ~ 35 %
  • Sodium phosphate balance

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H372

Hazard Classifications

STOT RE 1

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 5

1 of 5

Gentamicin solution 50 mg/mL in deionized water, liquid, 0.1 μm filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

G1397

Gentamicin solution

Sigma-Aldrich

Sigma-Aldrich

N6803

Nystatin water soluble

Voriconazole ≥98% (HPLC)

Sigma-Aldrich

PZ0005

Voriconazole

Gentamicin sulfate salt powder, BioReagent, suitable for cell culture

Sigma-Aldrich

G1264

Gentamicin sulfate salt

Penicillin G sodium salt powder, BioReagent, suitable for cell culture

Sigma-Aldrich

P3032

Penicillin G sodium salt

Devin Tran et al.
Cornea, 38(7), 901-904 (2019-04-19)
Fungal infections in lamellar keratoplasty are a growing concern. Optisol-GS does not contain an antifungal agent and supplementation with 0.255 μg/mL Amphotericin B (AmpB) has been considered. This study tested the ability of 0.255 μg/mL AmpB in Optisol-GS to eliminate
Moises A Huaman et al.
Frontiers in immunology, 11, 607957-607957 (2021-01-05)
Tuberculosis has been associated with increased risk of atherosclerotic cardiovascular disease. To examine whether mycobacterial infection exacerbates atherosclerosis development in experimental conditions, we infected low-density lipoprotein receptor knockout (Ldlr -/-) mice with Mycobacterium bovis Bacille-Calmette-Guérin (BCG), an attenuated strain of
Derayvia Grimes et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(39), 24403-24414 (2020-09-16)
Calcium signals are initiated in immune cells by the process of store-operated calcium entry (SOCE), where receptor activation triggers transient calcium release from the endoplasmic reticulum, followed by opening of plasma-membrane calcium-release activated calcium (CRAC) channels. ORAI1, ORAI2, and ORAI3
Elodie Fino et al.
PloS one, 4(8), e6557-e6557 (2009-08-14)
Action potentials are thought to be determinant for the induction of long-term synaptic plasticity, the cellular basis of learning and memory. However, neuronal activity does not lead systematically to an action potential but also, in many cases, to synaptic depolarizing
Malav S Trivedi et al.
The Journal of nutritional biochemistry, 25(10), 1011-1018 (2014-07-16)
Dietary interventions like gluten-free and casein-free diets have been reported to improve intestinal, autoimmune and neurological symptoms in patients with a variety of conditions; however, the underlying mechanism of benefit for such diets remains unclear. Epigenetic programming, including CpG methylation
View All Related Papers

Articles

Antifungals Reagents in Cell Culture

Prevent fungal, yeast, and mold contamination in cell cultures. Discover the best antifungal agent for your cultures with the extensive Sigma® antifungal collection.

Human Microbiome Research Workflow: An Overview of the Role in Human Health and Disease

An overview of human microbiome research, workflow challenges, sequencing, library production, data analysis, and available microbiome reagents to support your research.

Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service