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B3133

Sigma-Aldrich

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride

≥99% (TLC), suitable for substrate for trypsin

Synonym(s):

L-BAPA, L-BAPNA, BANI

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About This Item

Empirical Formula (Hill Notation):
C19H22N6O4 · HCl
CAS Number:
Molecular Weight:
434.88
Beilstein/REAXYS Number:
4081878
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32

Quality Level

assay

≥99% (TLC)

form

powder

solubility

acetone: water (1:1): 50 mg/mL

suitability

suitable for substrate for trypsin

storage temp.

−20°C

SMILES string

Cl[H].NC(=N)NCCC[C@H](NC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O

InChI

1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H/t16-;/m0./s1

InChI key

DEOKFPFLXFNAON-NTISSMGPSA-N

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General description

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride is a substrate for trypsin.

Application

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride has been used as a substrate:
  • for chymotrypsin and trypsin activity assay in enzymatic extract (EE) of M. tenellum and pancreatic tissue homogenates
  • in trypsin and papain standard curve generation
  • in serine protease and fibrinogenolytic assays of eupolytin1 protein

Linkage

Similar to B3279, but produced for Sigma.

Substrates

Chromogenic substrate for trypsin and other proteolytic enzymes.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Trypsin is an enzyme in the serine protease class that consists of a polypeptide chain of 223 amino acid residues. Multiple sources, grades and formulations of trypsin specifically designed for research applications are available.

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