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B932-05

Sigma-Aldrich

Bovine Endometrial Epithelial Cells: BEnEpC (Cryovial)

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

bovine endometrium

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

2n = 60

morphology

epithelial

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

infectious diseases

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

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BEnEpC provide an excellent model system to study many aspects of epithelial function and disease, especially those related to the endometrium and pregnancy-related signaling, such as seminal plasma induced prostaglandins F2α, (PGF2α), E2 (PGE2) and interleukin-6 release by endometrial cells (Norrby, 2012; Madej, 2012; Malgorzata, 2013), or the role of PLA in mediating oxytocin-stimulated production of prostaglandins (Tithof, 2007). BEnEpC have also been used to demonstrate the association between variants of gap junctions related genes and conception rate (Sugimoto, 2013), and to investigate cellular adherence and cytotoxicity of Histophilus somni (Hoshinoo, 2009).

Cell Line Origin

Uterus

Application

endometrial function, growth factor production, conception rate, cell adherence, uterine lining, maintenance of uterine patency, uterine contraction

Components

Bovine Endometrial Basal Media containing 10% FBS & 10% DMSO

Preparation Note

  • 2nd passage, >500,000 cells in Bovine Endometrial Basal Media containing 10% FBS & 10% DMSO
  • Can be cultured at least 10 doublings

Subculture Routine

Please refer to the BEnEpC Culture Protocol.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Protocols

Bovine Endometrial Endothelial Cells (BEnEpC) Culture Protocol

Technical information for working with bovine endometrial endothelial cells including thawing, subculturing and cryopreservation

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