BCK-EDU647
EdU-Click 647
sufficient for 100 assays
Synonym(s):
EdU Cell Proliferation Kit
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About This Item
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fluorescence
λex 641 nm; λem 663 nm
shipped in
wet ice
storage temp.
2-8°C
General description
EdU (5-ethynyl-2′-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis. In contrast to BrdU assays, the EdU-Click Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the Baseclick EdU-Click Assays utilize click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol reduces both the total number of steps and significantly decreases the total amount of time. The simple click chemistry detection procedure is complete within 30 minutes and is compatible with multiplexing for content and context-rich results.
Application
Imaging
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2 - Muta. 1B - Repr. 2
wgk_germany
WGK 3
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Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a
Related Content
Experimental studies measuring cell proliferation have had implications in cancer biology, immunology, cell biology, and developmental biology.
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